Aarhus University Seal / Aarhus Universitets segl

CBC-ARS2 stimulates 3'-end maturation of multiple RNA families and favors cap-proximal processing

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Documents

DOI

  • Marie Hallais, Equipe labellisée Ligue contre le Cancer, Institut de Génétique Moléculaire de Montpellier-Centre National de la Recherche Scientifique, Montpellier, France.
  • ,
  • Frédéric Pontvianne, Equipe labellisée Ligue contre le Cancer, Institut de Génétique Moléculaire de Montpellier–Centre National de la Recherche Scientifique, Montpellier
  • ,
  • Peter Refsing Andersen
  • Marcello Clerici, European Molecular Biology Laboratory Grenoble Outstation, Grenoble
  • ,
  • Daniela Lener, Equipe labellisée Ligue contre le Cancer, Institut de Génétique Moléculaire de Montpellier–Centre National de la Recherche Scientifique, Montpellier
  • ,
  • Nour El Houda Benbahouche, Equipe labellisée Ligue contre le Cancer, Institut de Génétique Moléculaire de Montpellier–Centre National de la Recherche Scientifique, Montpellier
  • ,
  • Thierry Gostan, Equipe labellisée Ligue contre le Cancer, Institut de Génétique Moléculaire de Montpellier–Centre National de la Recherche Scientifique, Montpellier
  • ,
  • Franck Vandermoere, Institut de Génomique Fonctionnelle, Centre National de la Recherche Scientifique, Montpellier
  • ,
  • Marie-Cécile Robert, Equipe labellisée Ligue contre le Cancer, Institut de Génétique Moléculaire de Montpellier–Centre National de la Recherche Scientifique, Montpellier
  • ,
  • Stephen Cusack, European Molecular Biology Laboratory Grenoble Outstation, Grenoble
  • ,
  • Céline Verheggen, Equipe labellisée Ligue contre le Cancer, Institut de Génétique Moléculaire de Montpellier–Centre National de la Recherche Scientifique, Montpellier
  • ,
  • Torben Heick Jensen
  • Edouard Bertrand, Equipe labellisée Ligue contre le Cancer, Institut de Génétique Moléculaire de Montpellier–Centre National de la Recherche Scientifique, Montpellier
The nuclear cap-binding complex (CBC) stimulates multiple steps in several RNA maturation pathways, but how it functions in humans is incompletely understood. For small, capped RNAs such as pre-snRNAs, the CBC recruits PHAX. Here, we identify the CBCAP complex, composed of CBC, ARS2 and PHAX, and show that both CBCAP and CBC-ARS2 complexes can be reconstituted from recombinant proteins. ARS2 stimulates PHAX binding to the CBC and snRNA 3'-end processing, thereby coupling maturation with export. In vivo, CBC and ARS2 bind similar capped noncoding and coding RNAs and stimulate their 3'-end processing. The strongest effects are for cap-proximal polyadenylation sites, and this favors premature transcription termination. ARS2 functions partly through the mRNA 3'-end cleavage factor CLP1, which binds RNA Polymerase II through PCF11. ARS2 is thus a major CBC effector that stimulates functional and cryptic 3'-end processing sites.
Original languageEnglish
JournalNature Structural and Molecular Biology
Volume20
Issue12
Pages (from-to)1358-1366
Number of pages9
ISSN1545-9993
DOIs
Publication statusPublished - 24 Nov 2013

See relations at Aarhus University Citationformats

Download statistics

No data available

ID: 68694885