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Catalase and NO CATALASE ACTIVITY1 Promote Autophagy-Dependent Cell Death in Arabidopsis

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  • Thomas Hackenberg
  • ,
  • Trine Juul
  • ,
  • Aija Auzina
  • ,
  • Sonia Gwizdz, Aarhus University, Denmark
  • Anna Malolepszy, Denmark
  • Katrien Van Der Kelen, Ghent University, Belgium
  • Svend Dam, Denmark
  • Simon Bressendorff, University of Copenhagen, Denmark
  • Andrea Maria Lorentzen, University of Southern Denmark, Denmark
  • Peter Roepstorff, Department of Biochemistry and Molecular Biology, University of Southern Denmark, Denmark
  • Kåre Lehmann Nielsen, Aalborg University, Denmark
  • Jan-Elo Jørgensen, Denmark
  • Daniel Hofius, University of Copenhagen, Denmark
  • Frank Van Breusegem, Ghent University, Belgium
  • Morten Petersen, University of Copenhagen, Denmark
  • Stig Uggerhøj Andersen
Programmed cell death often depends on generation of reactive oxygen species, which can be detoxified by antioxidative enzymes, including catalases. We previously isolated catalase-deficient mutants (cat2) in a screen for resistance to hydroxyurea-induced cell death. Here, we identify an Arabidopsis thaliana hydroxyurea-resistant autophagy mutant, atg2, which also shows reduced sensitivity to cell death triggered by the bacterial effector avrRpm1. To test if catalase deficiency likewise affected both hydroxyurea and avrRpm1 sensitivity, we selected mutants with extremely low catalase activities and showed that they carried mutations in a gene that we named NO CATALASE ACTIVITY1 (NCA1). nca1 mutants showed severely reduced activities of all three catalase isoforms in Arabidopsis, and loss of NCA1 function led to strong suppression of RPM1-triggered cell death. Basal and starvation-induced autophagy appeared normal in the nca1 and cat2 mutants. By contrast, autophagic degradation induced by avrRpm1 challenge was compromised, indicating that catalase acted upstream of immunity-triggered autophagy. The direct interaction of catalase with reactive oxygen species could allow catalase to act as a molecular link between reactive oxygen species and the promotion of autophagy-dependent cell death.
Original languageEnglish
JournalPlant Cell
Volume25
Issue11
Pages (from-to)4616-4626
Number of pages11
ISSN1040-4651
DOIs
Publication statusPublished - 27 Nov 2013

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