Capture of influenza by medullary dendritic cells via SIGN-R1 is essential for humoral immunity in draining lymph nodes

Santiago F. Gonzalez, Veronika Lukacs-Kornek, Michael P. Kuligowski, Lisa A. Pitcher, Søren Egedal Degn, Young-A Kim, Mary J. Cloninger, Luisa Martinez-Pomares, Siamon Gordon, Shannon J. Turley, Michael C. Carroll

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    Abstract

    A major pathway for B cell acquisition of lymph-borne particulate antigens relies on antigen capture by subcapsular sinus macrophages of the lymph node. Here we tested whether this mechanism is also important for humoral immunity to inactivated influenza virus. By multiple approaches, including multiphoton intravital imaging, we found that antigen capture by sinus-lining macrophages was important for limiting the systemic spread of virus but not for the generation of influenza-specific humoral immunity. Instead, we found that dendritic cells residing in the lymph node medulla use the lectin receptor SIGN-R1 to capture lymph-borne influenza virus and promote humoral immunity. Thus, our results have important implications for the generation of durable humoral immunity to viral pathogens through vaccination.
    Translated title of the contributionBinding af influenza af medulaere dendritiske celler via SIGN-R1 er essentielt for humoral immunitet i draenende lymfeknuder
    Original languageEnglish
    JournalNature Immunology
    Volume11
    Issue5
    Pages (from-to)427-435
    Number of pages9
    ISSN1529-2908
    Publication statusPublished - May 2010

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