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Capillary flow disturbances after experimental subarachnoid hemorrhage: a contributor to delayed cerebral ischemia?

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Capillary flow disturbances after experimental subarachnoid hemorrhage: a contributor to delayed cerebral ischemia? / Anzabi, Maryam; Angleys, Hugo; Aamand, Rasmus; Ardalan, Maryam; Mouridsen, Kim; Rasmussen, Peter Mondrup; Sørensen, Jens Christian Hedemann; Plesnila, Nikolaus; Østergaard, Leif; Iversen, Nina Kerting.

In: Microcirculation, Vol. 26, No. 3, e12516, 2019.

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@article{c8d638ad4d0b419e9ed74fdeb06f01c9,
title = "Capillary flow disturbances after experimental subarachnoid hemorrhage: a contributor to delayed cerebral ischemia?",
abstract = "BACKGROUND: The high mortality and morbidity after subarachnoid hemorrhage (SAH) is partly due to delayed cerebral ischemia, which is traditionally ascribed to development of angiographic vasospasms. This relation has been challenged, and capillary flow disturbances are proposed as another mechanism contributing to brain damage after SAH.OBJECTIVE: To investigate capillary flow changes four days following experimental SAH.METHODS: SAH was induced by endovascular perforation of circle of Willis. We used two-photon microscopy (TPM) to evaluate blood flow characteristics. Cortical capillary diameters were investigated by both TPM and histology.RESULTS: We found elevated capillary transit-time heterogeneity and mean transit time of blood in SAH mice compared to sham animals. We observed capillaries with stagnant red blood cells, and capillaries with increased red blood cell linear density in the SAH group, suggesting severe blood maldistribution among cortical capillaries. Favoring that these capillary flow changes were primary to upstream vasoconstrictions, TPM showed no significant differences in arteriolar diameter between groups, while histological examination showed reduced capillary diameter in SAH group.CONCLUSION: Our study shows profound subacute hypoperfusion and capillary flow disturbances in a mouse SAH model and suggests that these changes are the result of changes in capillary function, rather than upstream vasospasm. This article is protected by copyright. All rights reserved.",
keywords = "capillary transit time heterogeneity, delayed cerebral ischemia, microcirculation, subarachnoid hemorrhage, two-photon microscopy",
author = "Maryam Anzabi and Hugo Angleys and Rasmus Aamand and Maryam Ardalan and Kim Mouridsen and Rasmussen, {Peter Mondrup} and S{\o}rensen, {Jens Christian Hedemann} and Nikolaus Plesnila and Leif {\O}stergaard and Iversen, {Nina Kerting}",
note = "This article is protected by copyright. All rights reserved.",
year = "2019",
doi = "10.1111/micc.12516",
language = "English",
volume = "26",
journal = "Microcirculation",
issn = "1073-9688",
publisher = "JohnWiley & Sons Ltd.",
number = "3",

}

RIS

TY - JOUR

T1 - Capillary flow disturbances after experimental subarachnoid hemorrhage: a contributor to delayed cerebral ischemia?

AU - Anzabi, Maryam

AU - Angleys, Hugo

AU - Aamand, Rasmus

AU - Ardalan, Maryam

AU - Mouridsen, Kim

AU - Rasmussen, Peter Mondrup

AU - Sørensen, Jens Christian Hedemann

AU - Plesnila, Nikolaus

AU - Østergaard, Leif

AU - Iversen, Nina Kerting

N1 - This article is protected by copyright. All rights reserved.

PY - 2019

Y1 - 2019

N2 - BACKGROUND: The high mortality and morbidity after subarachnoid hemorrhage (SAH) is partly due to delayed cerebral ischemia, which is traditionally ascribed to development of angiographic vasospasms. This relation has been challenged, and capillary flow disturbances are proposed as another mechanism contributing to brain damage after SAH.OBJECTIVE: To investigate capillary flow changes four days following experimental SAH.METHODS: SAH was induced by endovascular perforation of circle of Willis. We used two-photon microscopy (TPM) to evaluate blood flow characteristics. Cortical capillary diameters were investigated by both TPM and histology.RESULTS: We found elevated capillary transit-time heterogeneity and mean transit time of blood in SAH mice compared to sham animals. We observed capillaries with stagnant red blood cells, and capillaries with increased red blood cell linear density in the SAH group, suggesting severe blood maldistribution among cortical capillaries. Favoring that these capillary flow changes were primary to upstream vasoconstrictions, TPM showed no significant differences in arteriolar diameter between groups, while histological examination showed reduced capillary diameter in SAH group.CONCLUSION: Our study shows profound subacute hypoperfusion and capillary flow disturbances in a mouse SAH model and suggests that these changes are the result of changes in capillary function, rather than upstream vasospasm. This article is protected by copyright. All rights reserved.

AB - BACKGROUND: The high mortality and morbidity after subarachnoid hemorrhage (SAH) is partly due to delayed cerebral ischemia, which is traditionally ascribed to development of angiographic vasospasms. This relation has been challenged, and capillary flow disturbances are proposed as another mechanism contributing to brain damage after SAH.OBJECTIVE: To investigate capillary flow changes four days following experimental SAH.METHODS: SAH was induced by endovascular perforation of circle of Willis. We used two-photon microscopy (TPM) to evaluate blood flow characteristics. Cortical capillary diameters were investigated by both TPM and histology.RESULTS: We found elevated capillary transit-time heterogeneity and mean transit time of blood in SAH mice compared to sham animals. We observed capillaries with stagnant red blood cells, and capillaries with increased red blood cell linear density in the SAH group, suggesting severe blood maldistribution among cortical capillaries. Favoring that these capillary flow changes were primary to upstream vasoconstrictions, TPM showed no significant differences in arteriolar diameter between groups, while histological examination showed reduced capillary diameter in SAH group.CONCLUSION: Our study shows profound subacute hypoperfusion and capillary flow disturbances in a mouse SAH model and suggests that these changes are the result of changes in capillary function, rather than upstream vasospasm. This article is protected by copyright. All rights reserved.

KW - capillary transit time heterogeneity

KW - delayed cerebral ischemia

KW - microcirculation

KW - subarachnoid hemorrhage

KW - two-photon microscopy

UR - http://www.scopus.com/inward/record.url?scp=85062693208&partnerID=8YFLogxK

U2 - 10.1111/micc.12516

DO - 10.1111/micc.12516

M3 - Journal article

C2 - 30431201

VL - 26

JO - Microcirculation

JF - Microcirculation

SN - 1073-9688

IS - 3

M1 - e12516

ER -