Bovine milk osteopontin - Targeting bacterial adhesion for biofilm control

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Self-performed mechanical tooth cleaning does usually not result in complete biofilm removal, due to the complex oral anatomy and the strong adhesion of the biofilm to the tooth. Therefore, different supportive measures are employed, most of which aim at the chemical eradication of bacteria in dental biofilms. As their bactericidal action impacts the entire oral microflora, agents that inhibit biofilm formation without killing bacteria, such as the bovine milk protein osteopontin, have gained increasing attention. Here, we investigate the adhesion of 8 bacterial species associated with dental caries to salivary-coated flow-cells in the presence or absence of osteopontin or the control protein caseinoglycomacropeptide (0.32 mM/L). After 1h of flow (9.45 mm/min) at 35 °C, adhering bacteria were quantified by digital image analysis in a total of 692 bright-field images. Experiments were performed in technical and biological duplicates. Bifidobacterium dentium, Rothia dentocariosa and Streptococcus mutans did not adhere to the flow cell, irrespective of the presence of osteopontin. Osteopontin reduced the adhesion of Actinomyces naeslundii, Actinomyces viscosus, Lactobacillus paracasei subsp. paracasei, Streptococcus mitis and Streptococcus oralis with 74.0%, 62.4%, 90.0%, 89.6% and 81.5%, respectively, compared to protein-free saliva. All reductions were statistically significant (p<0.05) and significantly stronger than the reductions observed for caseinoglycomacropeptide (p<0.05). ), as determined by two-sample t-tests. The broad range anti-adhesive effect of osteopontin on dental bacterial strains might explain the reduced biofilm formation observed and be exploited in vivo for increased caries control.
Original languageEnglish
Article number11
JournalCaries Research
Pages (from-to)185
Number of pages1
Publication statusPublished - 2016
EventORCA - 63rd Annual Congress of the European Organisation for Caries Research - Megaron Athens International Conference Centre, Athen, Greece
Duration: 6 Jul 20169 Jul 2016


ConferenceORCA - 63rd Annual Congress of the European Organisation for Caries Research
LocationMegaron Athens International Conference Centre
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