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Bovine Milk Oligosaccharides with Sialyllactose Improves Cognition in Preterm Pigs

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DOI

  • Karina Obelitz-Ryom, Comparative Pediatrics and Nutrition, Department of Veterinary and Animal Sciences, University of Copenhagen, 1870 Frederiksberg, Denmark. karina.ryom@sund.ku.dk.
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  • Stine Brandt Bering, Comparative Pediatrics and Nutrition, Department of Veterinary and Animal Sciences, University of Copenhagen, 1870 Frederiksberg, Denmark. sbb@sund.ku.dk.
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  • Silja Hvid Overgaard, Comparative Pediatrics and Nutrition, Department of Veterinary and Animal Sciences, University of Copenhagen, 1870 Frederiksberg, Denmark. siljahvid@msn.com.
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  • Simon Fristed Eskildsen
  • Steffen Ringgaard
  • Jonas Lynge Olesen
  • Kerstin Skovgaard, Department of Biotechnology and Biomedicine, The Technical University of Denmark, 2800 Lyngby, Denmark. kesk@dtu.dk.
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  • Stanislava Pankratova, Laboratory of Neural Plasticity, Department of Neuroscience, University of Copenhagen, 2200 København, Denmark. stasya@sund.ku.dk.
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  • Bing Wang, School of Animal & Veterinary Sciences, Charles Sturt University, Wagga 2678, Australia. biwang@csu.edu.au.
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  • Anders Brunse, Comparative Pediatrics and Nutrition, Department of Veterinary and Animal Sciences, University of Copenhagen, 1870 Frederiksberg, Denmark. anderss@sund.ku.dk.
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  • Anne Birgitte Heckmann, Arla Foods Ingredients, 8260 Viby, Denmark. AnneBirgitteLau.Heckmann@arlafoods.com.
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  • Martin Peter Rydal, Comparative Pediatrics and Nutrition, Department of Veterinary and Animal Sciences, University of Copenhagen, 1870 Frederiksberg, Denmark. martin.rydal@sund.ku.dk.
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  • Per Torp Sangild, Comparative Pediatrics and Nutrition, Department of Veterinary and Animal Sciences, University of Copenhagen, 1870 Frederiksberg, Denmark. pts@sund.ku.dk.
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  • Thomas Thymann, Comparative Pediatrics and Nutrition, Department of Veterinary and Animal Sciences, University of Copenhagen, 1870 Frederiksberg, Denmark. thomas.thymann@sund.ku.dk.

Optimal nutrition is important after preterm birth to facilitate normal brain development. Human milk is rich in sialic acid and preterm infants may benefit from supplementing formula with sialyllactose to support neurodevelopment. Using pigs as models, we hypothesized that sialyllactose supplementation improves brain development after preterm birth. Pigs (of either sex) were delivered by cesarean section at 90% gestation and fed a milk diet supplemented with either an oligosaccharide-enriched whey with sialyllactose (n = 20) or lactose (n = 20) for 19 days. Cognitive performance was tested in a spatial T-maze. Brains were collected for ex vivo magnetic resonance imaging (MRI), gene expression, and sialic acid measurements. For reference, term piglets (n = 14) were artificially reared under identical conditions and compared with vaginally born piglets naturally reared by the sow (n = 12). A higher proportion of sialyllactose supplemented preterm pigs reached the T-maze learning criteria relative to control preterm pigs (p < 0.05), and approximated the cognition level of term reference pigs (p < 0.01). Furthermore, supplemented pigs had upregulated genes related to sialic acid metabolism, myelination, and ganglioside biosynthesis in hippocampus. Sialyllactose supplementation did not lead to higher levels of sialic acid in the hippocampus or change MRI endpoints. Contrary, these parameters were strongly influenced by postconceptional age and postnatal rearing conditions. In conclusion, oligosaccharide-enriched whey with sialyllactose improved spatial cognition, with effects on hippocampal genes related to sialic acid metabolism, myelination, and ganglioside biosynthesis in preterm pigs. Dietary sialic acid enrichment may improve brain development in infants.

Original languageEnglish
Article number1335
JournalNutrients
Volume11
Issue6
Number of pages20
ISSN2072-6643
DOIs
Publication statusPublished - 14 Jun 2019

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