Bottom-up and top-down controls on Alteromonas macleodii lead to different dissolved organic matter compositions.

Qi Chen; Christian Lønborg; Feng Chen; Rui Zhang; Ruanhong Cai; Yunyun Li; Chen He; Quan Shi; Nianzhi Jiao; Qiang Zheng

doi:10.1093/ismeco/ycae010

Bottom-up and top-down controls on Alteromonas macleodii lead to different dissolved organic matter compositions.

Qi Chen, Christian Lønborg, Feng Chen, Rui Zhang, Ruanhong Cai, Yunyun Li, Chen He, Quan Shi, Nianzhi Jiao^*, Qiang Zheng^*

^*Corresponding author for this work

Department of Ecoscience - Marine Diversity and Experimental Ecology

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Abstract

The effects of both bottom-up (e.g. substrate) and top-down (e.g. viral lysis) controls on the molecular composition of dissolved organic matter have not been investigated. In this study, we investigated the dissolved organic matter composition of the model bacterium Alteromonas macleodii ATCC 27126 growing on different substrates (glucose, laminarin, extracts from a Synechococcus culture, oligotrophic seawater, and eutrophic seawater), and infected with a lytic phage. The ultra-high resolution mass spectrometry analysis showed that when growing on different substrates Alteromonas macleodii preferred to use reduced, saturated nitrogen-containing molecules (i.e. O4 formula species) and released or preserved oxidized, unsaturated sulfur-containing molecules (i.e. O7 formula species). However, when infected with the lytic phage, Alteromonas macleodii produced organic molecules with higher hydrogen saturation, and more nitrogen- or sulfur-containing molecules. Our results demonstrate that bottom-up (i.e. varying substrates) and top-down (i.e. viral lysis) controls leave different molecular fingerprints in the produced dissolved organic matter.

Original language	English
Article number	ycae010
Journal	ISME Communications
Volume	4
Issue	1
Number of pages	9
ISSN	2730-6151
DOIs	https://doi.org/10.1093/ismeco/ycae010
Publication status	Published - Jan 2024

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10.1093/ismeco/ycae010Licence: CC BY

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@article{147c3b2971d748859573f3d6cb0d8046,

title = "Bottom-up and top-down controls on Alteromonas macleodii lead to different dissolved organic matter compositions.",

abstract = "The effects of both bottom-up (e.g. substrate) and top-down (e.g. viral lysis) controls on the molecular composition of dissolved organic matter have not been investigated. In this study, we investigated the dissolved organic matter composition of the model bacterium Alteromonas macleodii ATCC 27126 growing on different substrates (glucose, laminarin, extracts from a Synechococcus culture, oligotrophic seawater, and eutrophic seawater), and infected with a lytic phage. The ultra-high resolution mass spectrometry analysis showed that when growing on different substrates Alteromonas macleodii preferred to use reduced, saturated nitrogen-containing molecules (i.e. O4 formula species) and released or preserved oxidized, unsaturated sulfur-containing molecules (i.e. O7 formula species). However, when infected with the lytic phage, Alteromonas macleodii produced organic molecules with higher hydrogen saturation, and more nitrogen- or sulfur-containing molecules. Our results demonstrate that bottom-up (i.e. varying substrates) and top-down (i.e. viral lysis) controls leave different molecular fingerprints in the produced dissolved organic matter. ",

author = "Qi Chen and Christian L{\o}nborg and Feng Chen and Rui Zhang and Ruanhong Cai and Yunyun Li and Chen He and Quan Shi and Nianzhi Jiao and Qiang Zheng",

note = "{\textcopyright} The Author(s) 2024. Published by Oxford University Press on behalf of the International Society for Microbial Ecology.",

year = "2024",

month = jan,

doi = "10.1093/ismeco/ycae010",

language = "English",

volume = "4",

journal = "ISME Communications",

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Bottom-up and top-down controls on Alteromonas macleodii lead to different dissolved organic matter compositions. / Chen, Qi; Lønborg, Christian; Chen, Feng et al.
In: ISME Communications, Vol. 4, No. 1, ycae010, 01.2024.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

TY - JOUR

T1 - Bottom-up and top-down controls on Alteromonas macleodii lead to different dissolved organic matter compositions.

AU - Chen, Qi

AU - Lønborg, Christian

AU - Chen, Feng

AU - Zhang, Rui

AU - Cai, Ruanhong

AU - Li, Yunyun

AU - He, Chen

AU - Shi, Quan

AU - Jiao, Nianzhi

AU - Zheng, Qiang

N1 - © The Author(s) 2024. Published by Oxford University Press on behalf of the International Society for Microbial Ecology.

PY - 2024/1

Y1 - 2024/1

N2 - The effects of both bottom-up (e.g. substrate) and top-down (e.g. viral lysis) controls on the molecular composition of dissolved organic matter have not been investigated. In this study, we investigated the dissolved organic matter composition of the model bacterium Alteromonas macleodii ATCC 27126 growing on different substrates (glucose, laminarin, extracts from a Synechococcus culture, oligotrophic seawater, and eutrophic seawater), and infected with a lytic phage. The ultra-high resolution mass spectrometry analysis showed that when growing on different substrates Alteromonas macleodii preferred to use reduced, saturated nitrogen-containing molecules (i.e. O4 formula species) and released or preserved oxidized, unsaturated sulfur-containing molecules (i.e. O7 formula species). However, when infected with the lytic phage, Alteromonas macleodii produced organic molecules with higher hydrogen saturation, and more nitrogen- or sulfur-containing molecules. Our results demonstrate that bottom-up (i.e. varying substrates) and top-down (i.e. viral lysis) controls leave different molecular fingerprints in the produced dissolved organic matter.

AB - The effects of both bottom-up (e.g. substrate) and top-down (e.g. viral lysis) controls on the molecular composition of dissolved organic matter have not been investigated. In this study, we investigated the dissolved organic matter composition of the model bacterium Alteromonas macleodii ATCC 27126 growing on different substrates (glucose, laminarin, extracts from a Synechococcus culture, oligotrophic seawater, and eutrophic seawater), and infected with a lytic phage. The ultra-high resolution mass spectrometry analysis showed that when growing on different substrates Alteromonas macleodii preferred to use reduced, saturated nitrogen-containing molecules (i.e. O4 formula species) and released or preserved oxidized, unsaturated sulfur-containing molecules (i.e. O7 formula species). However, when infected with the lytic phage, Alteromonas macleodii produced organic molecules with higher hydrogen saturation, and more nitrogen- or sulfur-containing molecules. Our results demonstrate that bottom-up (i.e. varying substrates) and top-down (i.e. viral lysis) controls leave different molecular fingerprints in the produced dissolved organic matter.

U2 - 10.1093/ismeco/ycae010

DO - 10.1093/ismeco/ycae010

M3 - Journal article

C2 - 38469454

SN - 2730-6151

VL - 4

JO - ISME Communications

JF - ISME Communications

IS - 1

M1 - ycae010

ER -

Bottom-up and top-down controls on Alteromonas macleodii lead to different dissolved organic matter compositions.

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