Blood ammonia concentration measurement - effects of sampling site and cirrhosis during induced hyperammonaemia

TY - JOUR

T1 - Blood ammonia concentration measurement - effects of sampling site and cirrhosis during induced hyperammonaemia

AU - Djernes, Lars

AU - Vilstrup, Hendrik

AU - Ott, Peter

AU - Eriksen, Peter Lykke

N1 - © 2024. The Author(s).

PY - 2025/1

Y1 - 2025/1

N2 - Background: Ammonia is implicated in hepatic encephalopathy (HE) and prognostic in cirrhosis. Venous ammonia concentration, yielding similar correlation with HE grades as arterial, has become the preferred practise but comparative data are limited. Aim: To quantify effect of sampling site on ammonia concentration in healthy persons and patients with cirrhosis. Methods: Ammonia concentrations were measured by arterial and femoral venous blood sampling in ten healthy men and ten male patients with cirrhosis before and during hyperammonaemia induced by ammonia infusion. Cubital vein samples were included during the infusion. Results: At baseline, arterial-venous concentration gaps were similar (p = 0.15) in healthy persons [14 (10–19) and 8 (4–12) µmol/L] and patients with cirrhosis [53 (32–74) and 40 (23–57) µmol/L]. Ammonia infusion increased arterial-venous concentration gaps in both groups [115 (97–133) and 61 (31–90) vs. 175 (123–227) and 134 (65–203) µmol/L]. Mean ammonia concentration difference between groups during hyperammonaemia was 72 (42–103) µmol/L (p < 0.001) and independent of sampling site. Cubital and femoral vein concentrations were comparable (p = 0.26). In cirrhosis, calculated upper limit normal values (ULN) were comparable for arterial and venous blood at baseline [2.0 (1.2–2.8) and 2.1 (1.2–3.0), p = 0.74] and during hyperammonaemia [6.7 (4.7–8.7) and 6.2 (4.4– 8.1), p = 0.44]. Conclusions: We found clinically meaningful intra-individual arterial-venous concentration gaps in both healthy persons and patients with cirrhosis at any ammonia concentration. Inter-group concentration differences after induced hyperammonaemia were relatively constant across sampling sites which supports clinical use of venous sampling. ULN-normalised ammonia concentrations were valid for both arterial and venous sampling.

AB - Background: Ammonia is implicated in hepatic encephalopathy (HE) and prognostic in cirrhosis. Venous ammonia concentration, yielding similar correlation with HE grades as arterial, has become the preferred practise but comparative data are limited. Aim: To quantify effect of sampling site on ammonia concentration in healthy persons and patients with cirrhosis. Methods: Ammonia concentrations were measured by arterial and femoral venous blood sampling in ten healthy men and ten male patients with cirrhosis before and during hyperammonaemia induced by ammonia infusion. Cubital vein samples were included during the infusion. Results: At baseline, arterial-venous concentration gaps were similar (p = 0.15) in healthy persons [14 (10–19) and 8 (4–12) µmol/L] and patients with cirrhosis [53 (32–74) and 40 (23–57) µmol/L]. Ammonia infusion increased arterial-venous concentration gaps in both groups [115 (97–133) and 61 (31–90) vs. 175 (123–227) and 134 (65–203) µmol/L]. Mean ammonia concentration difference between groups during hyperammonaemia was 72 (42–103) µmol/L (p < 0.001) and independent of sampling site. Cubital and femoral vein concentrations were comparable (p = 0.26). In cirrhosis, calculated upper limit normal values (ULN) were comparable for arterial and venous blood at baseline [2.0 (1.2–2.8) and 2.1 (1.2–3.0), p = 0.74] and during hyperammonaemia [6.7 (4.7–8.7) and 6.2 (4.4– 8.1), p = 0.44]. Conclusions: We found clinically meaningful intra-individual arterial-venous concentration gaps in both healthy persons and patients with cirrhosis at any ammonia concentration. Inter-group concentration differences after induced hyperammonaemia were relatively constant across sampling sites which supports clinical use of venous sampling. ULN-normalised ammonia concentrations were valid for both arterial and venous sampling.

KW - Adult

KW - Aged

KW - Ammonia/blood

KW - Hepatic Encephalopathy/blood

KW - Humans

KW - Hyperammonemia/blood

KW - Liver Cirrhosis/blood

KW - Male

KW - Middle Aged

KW - Sampling site

KW - Ammonia

KW - Cirrhosis

KW - Arterial-venous concentration differences

KW - Upper limit normal

UR - https://www.scopus.com/pages/publications/85209723785

U2 - 10.1007/s11011-024-01442-4

DO - 10.1007/s11011-024-01442-4

M3 - Journal article

C2 - 39565434

SN - 0885-7490

VL - 40

JO - Metabolic Brain Disease

JF - Metabolic Brain Disease

IS - 1

M1 - 21

ER -