‘‘Blind'' mapping of genic DNA sequence polymorphisms in Lolium perenne L. by high resolution melting curve analysis

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‘‘Blind'' mapping of genic DNA sequence polymorphisms in Lolium perenne L. by high resolution melting curve analysis. / Studer, Bruno; Jensen, Louise Bach; Fiil, Alice; Asp, Torben.

In: Molecular Breeding, Vol. 24, No. 2, 2009, p. 191-199.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

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Studer, Bruno ; Jensen, Louise Bach ; Fiil, Alice ; Asp, Torben. / ‘‘Blind'' mapping of genic DNA sequence polymorphisms in Lolium perenne L. by high resolution melting curve analysis. In: Molecular Breeding. 2009 ; Vol. 24, No. 2. pp. 191-199.

Bibtex

@article{505c1800c46411de8cbb000ea68e967b,
title = "‘‘Blind'' mapping of genic DNA sequence polymorphisms in Lolium perenne L. by high resolution melting curve analysis",
abstract = "High resolution melting curve analysis (HRM) measures dissociation of double stranded DNA of a PCR product amplified in the presence of a saturating fluorescence dye. Recently, HRM proved successful to genotype DNA sequence polymorphisms such as SSRs and SNPs based on the shape of the melting curves. In this study, HRM was used for simultaneous screening and genotyping of genic DNA sequence polymorphisms identified in the Lolium perenne F2 mapping population VrnA. Melting profiles of PCR products amplified from previously published gene loci and from a novel gene putatively involved in vernalization response successfully discriminated genotypes in absence of allelic sequence information, and allowed to determine allele segregation in VrnA. Here we introduce the concept of {"}blind{"} mapping based on HRM as a powerful, fast and cheap method to map any DNA sequence polymorphisms without prior knowledge of allelic sequences in the key grassland species perennial ryegrass (Lolium perenne L.).",
keywords = "“Blind” mapping, High resolution melting curve analysis (HRM), Linkage mapping, Lolium perenne L",
author = "Bruno Studer and Jensen, {Louise Bach} and Alice Fiil and Torben Asp",
year = "2009",
doi = "10.1007/s11032-009-9291-x",
language = "English",
volume = "24",
pages = "191--199",
journal = "Molecular Breeding",
issn = "1380-3743",
publisher = "Springer Netherlands",
number = "2",

}

RIS

TY - JOUR

T1 - ‘‘Blind'' mapping of genic DNA sequence polymorphisms in Lolium perenne L. by high resolution melting curve analysis

AU - Studer, Bruno

AU - Jensen, Louise Bach

AU - Fiil, Alice

AU - Asp, Torben

PY - 2009

Y1 - 2009

N2 - High resolution melting curve analysis (HRM) measures dissociation of double stranded DNA of a PCR product amplified in the presence of a saturating fluorescence dye. Recently, HRM proved successful to genotype DNA sequence polymorphisms such as SSRs and SNPs based on the shape of the melting curves. In this study, HRM was used for simultaneous screening and genotyping of genic DNA sequence polymorphisms identified in the Lolium perenne F2 mapping population VrnA. Melting profiles of PCR products amplified from previously published gene loci and from a novel gene putatively involved in vernalization response successfully discriminated genotypes in absence of allelic sequence information, and allowed to determine allele segregation in VrnA. Here we introduce the concept of "blind" mapping based on HRM as a powerful, fast and cheap method to map any DNA sequence polymorphisms without prior knowledge of allelic sequences in the key grassland species perennial ryegrass (Lolium perenne L.).

AB - High resolution melting curve analysis (HRM) measures dissociation of double stranded DNA of a PCR product amplified in the presence of a saturating fluorescence dye. Recently, HRM proved successful to genotype DNA sequence polymorphisms such as SSRs and SNPs based on the shape of the melting curves. In this study, HRM was used for simultaneous screening and genotyping of genic DNA sequence polymorphisms identified in the Lolium perenne F2 mapping population VrnA. Melting profiles of PCR products amplified from previously published gene loci and from a novel gene putatively involved in vernalization response successfully discriminated genotypes in absence of allelic sequence information, and allowed to determine allele segregation in VrnA. Here we introduce the concept of "blind" mapping based on HRM as a powerful, fast and cheap method to map any DNA sequence polymorphisms without prior knowledge of allelic sequences in the key grassland species perennial ryegrass (Lolium perenne L.).

KW - “Blind” mapping

KW - High resolution melting curve analysis (HRM)

KW - Linkage mapping

KW - Lolium perenne L

U2 - 10.1007/s11032-009-9291-x

DO - 10.1007/s11032-009-9291-x

M3 - Journal article

VL - 24

SP - 191

EP - 199

JO - Molecular Breeding

JF - Molecular Breeding

SN - 1380-3743

IS - 2

ER -