Biological transformation of fexofenadine and sitagliptin by carrier-attached biomass and suspended sludge from a hybrid moving bed biofilm reactor

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Biological transformation of fexofenadine and sitagliptin by carrier-attached biomass and suspended sludge from a hybrid moving bed biofilm reactor. / Henning, Nina; Falås, Per; Castronovo, Sandro; Jewell, Kevin S.; Bester, Kai; Ternes, Thomas A.; Wick, Arne.

In: Water Research, Vol. 167, 115034, 12.2019.

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Henning, Nina ; Falås, Per ; Castronovo, Sandro ; Jewell, Kevin S. ; Bester, Kai ; Ternes, Thomas A. ; Wick, Arne. / Biological transformation of fexofenadine and sitagliptin by carrier-attached biomass and suspended sludge from a hybrid moving bed biofilm reactor. In: Water Research. 2019 ; Vol. 167.

Bibtex

@article{ff5f4962e084434e9016d5d0d6b8c425,
title = "Biological transformation of fexofenadine and sitagliptin by carrier-attached biomass and suspended sludge from a hybrid moving bed biofilm reactor",
abstract = "Laboratory-scale experiments were conducted to investigate the (bio)transformation of the antidiabetic sitagliptin (STG) and the antihistamine fexofenadine (FXF) during wastewater treatment. As inoculum either attached-growth on carriers or suspended sludge from a hybrid moving bed biofilm reactor (HMBBR) was used. Both target compounds were incubated in degradation experiments and quantified via LC-MS/MS for degradation kinetics. Furthermore transformation products (TPs) were analyzed via high resolution mass spectrometry (HRMS). Structural elucidation of the TPs was based on the high resolution molecular ion mass to propose a molecular formula and on MS2 fragmentation to elucidate the chemical structure of the TPs. In total, 22 TPs (9 TPs for STG and 13 TPs for FXF) were detected in the experiments with STG and FXF. For all TPs, chemical structures could be proposed. STG was mainly transformed via amide hydrolysis and conjugation of the primary amine moiety. In contrast, FXF was predominantly transformed by oxidative reactions such as oxidation (dehydrogenation) and hydroxylation. Furthermore, FXF was removed significantly faster in contact with carriers compared to suspended sludge, whereas STG was degraded slightly faster in contact with suspended sludge. Moreover, the primary TP of FXF was also degraded faster in contact with carriers leading to higher proportions of secondary TPs. Thus, the microbial community of both carriers and suspended sludge catalyzed the same primary transformation reactions but the transformation kinetics of FXF and the formation/degradation of FXF TPs were considerably higher in contact with carrier-attached biomass. The primary degradation of both target compounds in pilot- and full-scale conventional activated sludge (CAS) and MBBR reactors reached 42 and 61% for FXF and STG, respectively. Up to three of the identified TPs of FXF and 8 TPs of STG were detected in the effluents of pilot- and full-scale CAS and MBBR.",
keywords = "ACTIVATED-SLUDGE, BIODEGRADATION, BIOTRANSFORMATION, Biotransformation, DRUGS, Fexofenadine, Moving bed biofilm reactor, PHARMACEUTICALS, POLAR ORGANIC MICROPOLLUTANTS, PRODUCTS, REMOVAL, Sitagliptin, TREATMENT PLANTS, Transformation products, WASTE-WATER TREATMENT",
author = "Nina Henning and Per Fal{\aa}s and Sandro Castronovo and Jewell, {Kevin S.} and Kai Bester and Ternes, {Thomas A.} and Arne Wick",
year = "2019",
month = dec,
doi = "10.1016/j.watres.2019.115034",
language = "English",
volume = "167",
journal = "Water Research",
issn = "0043-1354",
publisher = "I W A Publishing",

}

RIS

TY - JOUR

T1 - Biological transformation of fexofenadine and sitagliptin by carrier-attached biomass and suspended sludge from a hybrid moving bed biofilm reactor

AU - Henning, Nina

AU - Falås, Per

AU - Castronovo, Sandro

AU - Jewell, Kevin S.

AU - Bester, Kai

AU - Ternes, Thomas A.

AU - Wick, Arne

PY - 2019/12

Y1 - 2019/12

N2 - Laboratory-scale experiments were conducted to investigate the (bio)transformation of the antidiabetic sitagliptin (STG) and the antihistamine fexofenadine (FXF) during wastewater treatment. As inoculum either attached-growth on carriers or suspended sludge from a hybrid moving bed biofilm reactor (HMBBR) was used. Both target compounds were incubated in degradation experiments and quantified via LC-MS/MS for degradation kinetics. Furthermore transformation products (TPs) were analyzed via high resolution mass spectrometry (HRMS). Structural elucidation of the TPs was based on the high resolution molecular ion mass to propose a molecular formula and on MS2 fragmentation to elucidate the chemical structure of the TPs. In total, 22 TPs (9 TPs for STG and 13 TPs for FXF) were detected in the experiments with STG and FXF. For all TPs, chemical structures could be proposed. STG was mainly transformed via amide hydrolysis and conjugation of the primary amine moiety. In contrast, FXF was predominantly transformed by oxidative reactions such as oxidation (dehydrogenation) and hydroxylation. Furthermore, FXF was removed significantly faster in contact with carriers compared to suspended sludge, whereas STG was degraded slightly faster in contact with suspended sludge. Moreover, the primary TP of FXF was also degraded faster in contact with carriers leading to higher proportions of secondary TPs. Thus, the microbial community of both carriers and suspended sludge catalyzed the same primary transformation reactions but the transformation kinetics of FXF and the formation/degradation of FXF TPs were considerably higher in contact with carrier-attached biomass. The primary degradation of both target compounds in pilot- and full-scale conventional activated sludge (CAS) and MBBR reactors reached 42 and 61% for FXF and STG, respectively. Up to three of the identified TPs of FXF and 8 TPs of STG were detected in the effluents of pilot- and full-scale CAS and MBBR.

AB - Laboratory-scale experiments were conducted to investigate the (bio)transformation of the antidiabetic sitagliptin (STG) and the antihistamine fexofenadine (FXF) during wastewater treatment. As inoculum either attached-growth on carriers or suspended sludge from a hybrid moving bed biofilm reactor (HMBBR) was used. Both target compounds were incubated in degradation experiments and quantified via LC-MS/MS for degradation kinetics. Furthermore transformation products (TPs) were analyzed via high resolution mass spectrometry (HRMS). Structural elucidation of the TPs was based on the high resolution molecular ion mass to propose a molecular formula and on MS2 fragmentation to elucidate the chemical structure of the TPs. In total, 22 TPs (9 TPs for STG and 13 TPs for FXF) were detected in the experiments with STG and FXF. For all TPs, chemical structures could be proposed. STG was mainly transformed via amide hydrolysis and conjugation of the primary amine moiety. In contrast, FXF was predominantly transformed by oxidative reactions such as oxidation (dehydrogenation) and hydroxylation. Furthermore, FXF was removed significantly faster in contact with carriers compared to suspended sludge, whereas STG was degraded slightly faster in contact with suspended sludge. Moreover, the primary TP of FXF was also degraded faster in contact with carriers leading to higher proportions of secondary TPs. Thus, the microbial community of both carriers and suspended sludge catalyzed the same primary transformation reactions but the transformation kinetics of FXF and the formation/degradation of FXF TPs were considerably higher in contact with carrier-attached biomass. The primary degradation of both target compounds in pilot- and full-scale conventional activated sludge (CAS) and MBBR reactors reached 42 and 61% for FXF and STG, respectively. Up to three of the identified TPs of FXF and 8 TPs of STG were detected in the effluents of pilot- and full-scale CAS and MBBR.

KW - ACTIVATED-SLUDGE

KW - BIODEGRADATION

KW - BIOTRANSFORMATION

KW - Biotransformation

KW - DRUGS

KW - Fexofenadine

KW - Moving bed biofilm reactor

KW - PHARMACEUTICALS

KW - POLAR ORGANIC MICROPOLLUTANTS

KW - PRODUCTS

KW - REMOVAL

KW - Sitagliptin

KW - TREATMENT PLANTS

KW - Transformation products

KW - WASTE-WATER TREATMENT

UR - http://www.scopus.com/inward/record.url?scp=85069557830&partnerID=8YFLogxK

U2 - 10.1016/j.watres.2019.115034

DO - 10.1016/j.watres.2019.115034

M3 - Journal article

C2 - 31581038

AN - SCOPUS:85069557830

VL - 167

JO - Water Research

JF - Water Research

SN - 0043-1354

M1 - 115034

ER -