Biological activity and biotechnological aspects of locked nucleic acids

Karin E Lundin; Torben Højland; Bo Hansen; Robert Persson; Jesper B Bramsen; Jørgen Kjems; Troels Koch; Jesper Wengel; C I Edvard Smith

doi:10.1016/B978-0-12-407676-1.00002-0

Biological activity and biotechnological aspects of locked nucleic acids

Karin E Lundin, Torben Højland, Bo Hansen, Robert Persson, Jesper B Bramsen, Jørgen Kjems, Troels Koch, Jesper Wengel, C I Edvard Smith

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

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Abstract

Locked nucleic acid (LNA) is one of the most promising new nucleic acid analogues that has been produced under the past two decades. In this chapter, we have tried to cover many of the different areas, where this molecule has been used to improve the function of synthetic oligonucleotides (ONs). The use of LNA in antisense ONs, including gapmers, splice-switching ONs, and siLNA, as well as antigene ONs, is reviewed. Pharmacokinetics as well as pharmacodynamics of LNA ONs and a description of selected compounds in, or close to, clinical testing are described. In addition, new LNA modifications and the adaptation of enzymes for LNA incorporation are reviewed. Such enzymes may become important for the development of stabilized LNA-containing aptamers.

Original language	English
Book series	Advances in Genetics
Volume	82
Pages (from-to)	47-107
Number of pages	61
ISSN	0065-2660
DOIs	https://doi.org/10.1016/B978-0-12-407676-1.00002-0
Publication status	Published - 2013

Keywords

Animals
Aptamers, Nucleotide
DNA, Antisense
Humans
MicroRNAs
Oligonucleotides
RNA Splicing
RNA, Small Interfering

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10.1016/B978-0-12-407676-1.00002-0

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title = "Biological activity and biotechnological aspects of locked nucleic acids",

abstract = "Locked nucleic acid (LNA) is one of the most promising new nucleic acid analogues that has been produced under the past two decades. In this chapter, we have tried to cover many of the different areas, where this molecule has been used to improve the function of synthetic oligonucleotides (ONs). The use of LNA in antisense ONs, including gapmers, splice-switching ONs, and siLNA, as well as antigene ONs, is reviewed. Pharmacokinetics as well as pharmacodynamics of LNA ONs and a description of selected compounds in, or close to, clinical testing are described. In addition, new LNA modifications and the adaptation of enzymes for LNA incorporation are reviewed. Such enzymes may become important for the development of stabilized LNA-containing aptamers.",

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author = "Lundin, {Karin E} and Torben H{\o}jland and Bo Hansen and Robert Persson and Bramsen, {Jesper B} and J{\o}rgen Kjems and Troels Koch and Jesper Wengel and Smith, {C I Edvard}",

year = "2013",

doi = "10.1016/B978-0-12-407676-1.00002-0",

language = "English",

volume = "82",

pages = "47--107",

journal = "Advances in Genetics",

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TY - JOUR

T1 - Biological activity and biotechnological aspects of locked nucleic acids

AU - Lundin, Karin E

AU - Højland, Torben

AU - Hansen, Bo

AU - Persson, Robert

AU - Bramsen, Jesper B

AU - Kjems, Jørgen

AU - Koch, Troels

AU - Wengel, Jesper

AU - Smith, C I Edvard

PY - 2013

Y1 - 2013

N2 - Locked nucleic acid (LNA) is one of the most promising new nucleic acid analogues that has been produced under the past two decades. In this chapter, we have tried to cover many of the different areas, where this molecule has been used to improve the function of synthetic oligonucleotides (ONs). The use of LNA in antisense ONs, including gapmers, splice-switching ONs, and siLNA, as well as antigene ONs, is reviewed. Pharmacokinetics as well as pharmacodynamics of LNA ONs and a description of selected compounds in, or close to, clinical testing are described. In addition, new LNA modifications and the adaptation of enzymes for LNA incorporation are reviewed. Such enzymes may become important for the development of stabilized LNA-containing aptamers.

AB - Locked nucleic acid (LNA) is one of the most promising new nucleic acid analogues that has been produced under the past two decades. In this chapter, we have tried to cover many of the different areas, where this molecule has been used to improve the function of synthetic oligonucleotides (ONs). The use of LNA in antisense ONs, including gapmers, splice-switching ONs, and siLNA, as well as antigene ONs, is reviewed. Pharmacokinetics as well as pharmacodynamics of LNA ONs and a description of selected compounds in, or close to, clinical testing are described. In addition, new LNA modifications and the adaptation of enzymes for LNA incorporation are reviewed. Such enzymes may become important for the development of stabilized LNA-containing aptamers.

KW - Animals

KW - Aptamers, Nucleotide

KW - DNA, Antisense

KW - Humans

KW - MicroRNAs

KW - Oligonucleotides

KW - RNA Splicing

KW - RNA, Small Interfering

U2 - 10.1016/B978-0-12-407676-1.00002-0

DO - 10.1016/B978-0-12-407676-1.00002-0

M3 - Journal article

C2 - 23721720

SN - 0065-2660

VL - 82

SP - 47

EP - 107

JO - Advances in Genetics

JF - Advances in Genetics

ER -

Biological activity and biotechnological aspects of locked nucleic acids

Abstract

Keywords

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