Bioimaging of teeth and their surrounding tissues and biofilm

TY - CONF

T1 - Bioimaging of teeth and their surrounding tissues and biofilm

AU - Dige, Irene

AU - Spin-Neto, Rubens

AU - Kraft, David Christian Evar

PY - 2017/11/1

Y1 - 2017/11/1

N2 - At the Department of Dentistry and Oral Health, bioimaging is a central part of our research of dental tissues and diseases in the oral cavity. We conduct research in the understanding, preventing, and treating of such diseases and there has been a strategic focus on the image-based investigation of clinical problems. For example, because of the etiological role of biofilms in many diseases including dental caries and periodontitis, we have investigated biofilm ecology combining newer molecular techniques such as Confocal Laser Scanning Microscopy (CLSM) and fluorescence techniques. These methods offer new opportunities for analyzing bacterial population dynamics and dental biofilm architecture. Recently, we have developed a novel pH-ratiometric-method using CLSM together with a fluorescent dye which allows us to define ecological micro-niches within intact biofilms. Radiographic imaging methods are also used within Dentistry. Especial attention is given to those methods allowing the three-dimensional visualization of the mineralized structures of the maxillo-facial region, being the most relevant cone-beam CT (for in vivo imaging) and micro-CT (for ex vivo and in vitro imaging). For in vivo investigations, to keep the radiation dose to the lowest achievable, images with submillimeter resolution are a reality. For those investigations in which the radiation dose is not relevant, the achievable resolution is higher. Although advances, has been made, within the field of radiographic imaging, basic histology is still the gold standard for study of the detailed anatomy of hard and soft tissue. We investigate outcome in both calcified and decalcified tissue, using saw microtome, sliding POLYCUT microtome and rotary microtome. Imaging of tissue sections have been greatly improved by new technologies allowing rapid creation of high-resolution panoramic images that allow digital analysis. Several of the methods used can be used in research fields outside the dental field, and interdisciplinary collaborations are welcome to strengthen such possibility.

AB - At the Department of Dentistry and Oral Health, bioimaging is a central part of our research of dental tissues and diseases in the oral cavity. We conduct research in the understanding, preventing, and treating of such diseases and there has been a strategic focus on the image-based investigation of clinical problems. For example, because of the etiological role of biofilms in many diseases including dental caries and periodontitis, we have investigated biofilm ecology combining newer molecular techniques such as Confocal Laser Scanning Microscopy (CLSM) and fluorescence techniques. These methods offer new opportunities for analyzing bacterial population dynamics and dental biofilm architecture. Recently, we have developed a novel pH-ratiometric-method using CLSM together with a fluorescent dye which allows us to define ecological micro-niches within intact biofilms. Radiographic imaging methods are also used within Dentistry. Especial attention is given to those methods allowing the three-dimensional visualization of the mineralized structures of the maxillo-facial region, being the most relevant cone-beam CT (for in vivo imaging) and micro-CT (for ex vivo and in vitro imaging). For in vivo investigations, to keep the radiation dose to the lowest achievable, images with submillimeter resolution are a reality. For those investigations in which the radiation dose is not relevant, the achievable resolution is higher. Although advances, has been made, within the field of radiographic imaging, basic histology is still the gold standard for study of the detailed anatomy of hard and soft tissue. We investigate outcome in both calcified and decalcified tissue, using saw microtome, sliding POLYCUT microtome and rotary microtome. Imaging of tissue sections have been greatly improved by new technologies allowing rapid creation of high-resolution panoramic images that allow digital analysis. Several of the methods used can be used in research fields outside the dental field, and interdisciplinary collaborations are welcome to strengthen such possibility.

M3 - Poster

T2 - Danish Bioimaging Scientific Event

Y2 - 8 November 2017 through 8 November 2017

ER -