Bioadhesive anisotropic nanogrooved microfibers directing three-dimensional neurite extension

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review


  • Yilin Chen
  • ,
  • Mehmet Berat Taskin
  • ,
  • Zhongyang Zhang
  • Yingchun Su
  • Xiaojun Han, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, 92 West Da-Zhi Street, Harbin, 150001, China.
  • ,
  • Menglin Chen

Neurodegenerative diseases and acute nerve injuries are becoming global clinical problems. Engineering three-dimensional (3D), anisotropic neural cellular structures in vitro is therefore desirable in the regenerative medicine research community. Here, we present, for the first time, a single-step, facile but delicate, fabrication of a 3D macroporous microfibrous scaffold with both anisotropic nanogrooved topography and in situ functionalization with a mussel inspired bioadhesive, poly(norepinephrine) (pNE). Specifically, immiscible blends of polycaprolactone (PCL) and polyethylene oxide (PEO) were electrospun into a grounded coagulation bath containing the precursor of pNE. Upon jet entrance in the bath, both phase-separation-driven longitudinal nanotopography and in situ pNE surface functionalization were introduced on individual microfibers that were packed into a 3D macroporous structure. The resulting scaffold significantly promoted 3D neurite extension capacity, 8-fold higher neurite extension over the isotropic counterpart, demonstrating that such a scaffold has great promise in 3D neural cell culture for nerve tissue modelling and engineering.

Original languageEnglish
JournalBiomaterials Science
Pages (from-to)2165-2173
Number of pages9
Publication statusPublished - May 2019

    Research areas

  • Adhesiveness, Animals, Anisotropy, Biocompatible Materials/chemistry, Electricity, Materials Testing, Nanotechnology, Neurites/drug effects, PC12 Cells, Polyesters/chemistry, Polyethylene Glycols/chemistry, Porosity, Rats

See relations at Aarhus University Citationformats

ID: 148223686