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Bioactive Nano-fibrous Scaffold for Vascularized Craniofacial Bone Regeneration

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  • Rahul Damodaran Prabha, Odense University Hospital
  • ,
  • David Christian Evar Kraft
  • Linda Harkness, Odense University Hospital, Denmark
  • Birte Melsen
  • ,
  • Harikrishna Varma, Sree Chitra Tirunal Institute for Medical Sciences and Technology (SCTIMST),Thiruvananthapuram, Kerala, India, India
  • Prabha D Nair, Sree Chitra Tirunal Institute for Medical Sciences and Technology (SCTIMST),Thiruvananthapuram, Kerala, India, India
  • Jorgen Kjems
  • Mousthapha Kassem, Odense University Hospital, Denmark

There has been a growing demand for bone grafts for correction of bone defects in complicated fractures or tumors in the craniofacial region. Soft flexible membrane like material that could be inserted into defect by less invasive approaches; promote osteoconductivity and act as a barrier to soft tissue in growth while promoting bone formation is an attractive option for this region. Electrospinning has recently emerged as one of the most promising techniques for fabrication of extracellular matrix (ECM) like nano-fibrous scaffolds that can serve as a template for bone formation. To overcome the limitation of cell penetration of electrospun scaffolds and improve on its osteoconductive nature, in this study, we fabricated a novel electrospun composite scaffold of polyvinyl alcohol (PVA) - poly (ε) caprolactone (PCL) - Bioceramic (HAB), namely, PVA-PCL-HAB. The scaffold prepared by dual electrospinning of PVA and PCL with HAB overcomes reduced cell attachment associated with hydrophobic poly (ε) caprolactone (PCL) by combination with a hydrophilic polyvinyl alcohol (PVA) and the bioceramic (HAB) can contribute to enhance osteo-conductivity. We characterized the physicochemical and biocompatibility properties of the new scaffold material. Our results indicate PVA-PCL-HAB scaffolds support attachment and growth of stromal stem cells; (human bone marrow skeletal (mesenchymal) stem cells (hMSC) and dental pulp stem cells (DPSC)). In addition, the scaffold supported in vitro osteogenic differentiation and in vivo vascularized bone formation. Thus, PVA-PCL-HAB scaffold is a suitable potential material for therapeutic bone regeneration in dentistry and orthopaedics.

Original languageEnglish
JournalJournal of Tissue Engineering and Regenerative Medicine
Pages (from-to)e1537-e1548
Number of pages12
Publication statusPublished - Mar 2018

    Research areas

  • bioceramics, bone, craniofacial, electrospinning, scaffold, stem cells

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