Binding of IFT22 to the intraflagellar transport complex is essential for flagellum assembly

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Binding of IFT22 to the intraflagellar transport complex is essential for flagellum assembly. / Wachter, Stefanie; Jung, Jamin; Shafiq, Shahaan; Basquin, Jerome; Fort, Cécile; Bastin, Philippe; Lorentzen, Esben.

In: EMBO Journal, Vol. 38, No. 9, e101251, 05.2019.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

Author

Wachter, Stefanie ; Jung, Jamin ; Shafiq, Shahaan ; Basquin, Jerome ; Fort, Cécile ; Bastin, Philippe ; Lorentzen, Esben. / Binding of IFT22 to the intraflagellar transport complex is essential for flagellum assembly. In: EMBO Journal. 2019 ; Vol. 38, No. 9.

Bibtex

@article{bf56b59089884b18945f3fc1caf7d895,

title = "Binding of IFT22 to the intraflagellar transport complex is essential for flagellum assembly",

abstract = "Intraflagellar transport (IFT) relies on motor proteins and the IFT complex to construct cilia and flagella. The IFT complex subunit IFT22/RabL5 has sequence similarity with small GTPases although the nucleotide specificity is unclear because of non-conserved G4/G5 motifs. We show that IFT22 specifically associates with G-nucleotides and present crystal structures of IFT22 in complex with GDP, GTP, and with IFT74/81. Our structural analysis unravels an unusual GTP/GDP-binding mode of IFT22 bypassing the classical G4 motif. The GTPase switch regions of IFT22 become ordered upon complex formation with IFT74/81 and mediate most of the IFT22-74/81 interactions. Structure-based mutagenesis reveals that association of IFT22 with the IFT complex is essential for flagellum construction in Trypanosoma brucei although IFT22 GTP-loading is not strictly required.",

keywords = "cilia, GTPase, IFT22, intraflagellar transport, Trypanosoma brucei",

author = "Stefanie Wachter and Jamin Jung and Shahaan Shafiq and Jerome Basquin and C{\'e}cile Fort and Philippe Bastin and Esben Lorentzen",

year = "2019",

month = may,

doi = "10.15252/embj.2018101251",

language = "English",

volume = "38",

journal = "E M B O Journal",

issn = "0261-4189",

publisher = "Wiley-Blackwell Publishing Ltd.",

number = "9",

}

RIS

TY - JOUR

T1 - Binding of IFT22 to the intraflagellar transport complex is essential for flagellum assembly

AU - Wachter, Stefanie

AU - Jung, Jamin

AU - Shafiq, Shahaan

AU - Basquin, Jerome

AU - Fort, Cécile

AU - Bastin, Philippe

AU - Lorentzen, Esben

PY - 2019/5

Y1 - 2019/5

N2 - Intraflagellar transport (IFT) relies on motor proteins and the IFT complex to construct cilia and flagella. The IFT complex subunit IFT22/RabL5 has sequence similarity with small GTPases although the nucleotide specificity is unclear because of non-conserved G4/G5 motifs. We show that IFT22 specifically associates with G-nucleotides and present crystal structures of IFT22 in complex with GDP, GTP, and with IFT74/81. Our structural analysis unravels an unusual GTP/GDP-binding mode of IFT22 bypassing the classical G4 motif. The GTPase switch regions of IFT22 become ordered upon complex formation with IFT74/81 and mediate most of the IFT22-74/81 interactions. Structure-based mutagenesis reveals that association of IFT22 with the IFT complex is essential for flagellum construction in Trypanosoma brucei although IFT22 GTP-loading is not strictly required.

AB - Intraflagellar transport (IFT) relies on motor proteins and the IFT complex to construct cilia and flagella. The IFT complex subunit IFT22/RabL5 has sequence similarity with small GTPases although the nucleotide specificity is unclear because of non-conserved G4/G5 motifs. We show that IFT22 specifically associates with G-nucleotides and present crystal structures of IFT22 in complex with GDP, GTP, and with IFT74/81. Our structural analysis unravels an unusual GTP/GDP-binding mode of IFT22 bypassing the classical G4 motif. The GTPase switch regions of IFT22 become ordered upon complex formation with IFT74/81 and mediate most of the IFT22-74/81 interactions. Structure-based mutagenesis reveals that association of IFT22 with the IFT complex is essential for flagellum construction in Trypanosoma brucei although IFT22 GTP-loading is not strictly required.

KW - cilia

KW - GTPase

KW - IFT22

KW - intraflagellar transport

KW - Trypanosoma brucei

UR - http://www.scopus.com/inward/record.url?scp=85063799499&partnerID=8YFLogxK

U2 - 10.15252/embj.2018101251

DO - 10.15252/embj.2018101251

M3 - Journal article

C2 - 30940671

AN - SCOPUS:85063799499

VL - 38

JO - E M B O Journal

JF - E M B O Journal

SN - 0261-4189

IS - 9

M1 - e101251

ER -