Herman Autrup, Human Tissue Studies Section, Experimental Pathology Branch, Division of Cancer Cause and Prevention, NCI, National Institutes of Health, Public Health Service, U.S. Department of Health, Education, and Welfare, Bethesda, Maryland, Denmark
C.C. Harris, United States
G.D. Stoner, United States
M.L. Jesudason, United States
B.F. Trump, United States
Institute of Environmental and Occupational Medicine
Metabolic activation of different chemical classes of carcinogens was studied in cultured human colon epithelia. Human colon epithelia were maintained in explant culture up to 4 days. Binding of benzo(a)pyrene, dimethylnitrosamine, and 1,2- dimethylhydrazine was found in both cell DNA and protein. 1,2-Dimethylhydrazine methylated DNA at both N·7 and 0-6 positions of guanin.
