Bicarbonate as tracer for plant assimilated C and homogeneity of 14C and 15N distribution in ryegrass and white clover tissue by alternative labeling approaches.

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Bicarbonate as tracer for plant assimilated C and homogeneity of 14C and 15N distribution in ryegrass and white clover tissue by alternative labeling approaches. / Rasmussen, Jim; Kusliene, Gedrime; Jacobsen, Ole Stig; Kuzyakov, Yakov; Eriksen, Jørgen.

In: Plant and Soil, Vol. 371, No. 1-2, 2013, p. 191-198.

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@article{10017c5d4aca4b70abb17eceeab2bef3,
title = "Bicarbonate as tracer for plant assimilated C and homogeneity of 14C and 15N distribution in ryegrass and white clover tissue by alternative labeling approaches.",
abstract = "Aims: Application of carbon (C) and nitrogen (N) isotopes is an essential tool to study C and N flows in plant-soil-microorganisms systems. When targeting single plants in a community the tracers need to be added via e.g., leaf-labeling or stem-feeding approaches. In this study we: (i) investigated if bicarbonate can be used to introduce 14C (or 13C) into white clover and ryegrass, and (ii) compared the patterns of 14C and 15N allocation in white clover and ryegrass to evaluate the homogeneity of tracer distribution after two alternative labeling approaches.MethodsPerennial ryegrass and white clover were pulse labeled with 15N urea via leaf-labeling and 14C either via a 14CO2 atm or with 14C bicarbonate through leaf-labeling. Plants were sampled 4 days after labeling and prepared for bulk isotope analysis and for 14C imaging to identify plant parts with high and low 14C activity. Subsequently, plant parts with high and low 14C activity were separated and analyzed for 15N enrichment.ResultsBicarbonate applied by leaf-labeling efficiently introduced 14C into both white clover and ryegrass, although the 14C activity in particular for white clover was found predominantly in the labeled leaf. Using 14C imaging for identification of areas with high (hotspots) and low 14C activity showed that 14C was incorporated very heterogeneously both when using bicarbonate and CO2 as expected when using pulse labeling. Subsequent analysis of 15N enrichment in plant parts with high and low 14C activity showed that 15N also had a heterogeneous distribution (up to two orders of magnitude).ConclusionBicarbonate can efficiently be used to introduce 14C or 13C into plant via the leaf-labeling method. Both 14C and 15N showed heterogeneous distribution in the plant, although the distribution of 15N was more even than that of 14C.",
author = "Jim Rasmussen and Gedrime Kusliene and Jacobsen, {Ole Stig} and Yakov Kuzyakov and J{\o}rgen Eriksen",
year = "2013",
doi = "10.1007/s11104-013-1660-x",
language = "English",
volume = "371",
pages = "191--198",
journal = "Plant and Soil",
issn = "0032-079X",
publisher = "Springer Netherlands",
number = "1-2",

}

RIS

TY - JOUR

T1 - Bicarbonate as tracer for plant assimilated C and homogeneity of 14C and 15N distribution in ryegrass and white clover tissue by alternative labeling approaches.

AU - Rasmussen, Jim

AU - Kusliene, Gedrime

AU - Jacobsen, Ole Stig

AU - Kuzyakov, Yakov

AU - Eriksen, Jørgen

PY - 2013

Y1 - 2013

N2 - Aims: Application of carbon (C) and nitrogen (N) isotopes is an essential tool to study C and N flows in plant-soil-microorganisms systems. When targeting single plants in a community the tracers need to be added via e.g., leaf-labeling or stem-feeding approaches. In this study we: (i) investigated if bicarbonate can be used to introduce 14C (or 13C) into white clover and ryegrass, and (ii) compared the patterns of 14C and 15N allocation in white clover and ryegrass to evaluate the homogeneity of tracer distribution after two alternative labeling approaches.MethodsPerennial ryegrass and white clover were pulse labeled with 15N urea via leaf-labeling and 14C either via a 14CO2 atm or with 14C bicarbonate through leaf-labeling. Plants were sampled 4 days after labeling and prepared for bulk isotope analysis and for 14C imaging to identify plant parts with high and low 14C activity. Subsequently, plant parts with high and low 14C activity were separated and analyzed for 15N enrichment.ResultsBicarbonate applied by leaf-labeling efficiently introduced 14C into both white clover and ryegrass, although the 14C activity in particular for white clover was found predominantly in the labeled leaf. Using 14C imaging for identification of areas with high (hotspots) and low 14C activity showed that 14C was incorporated very heterogeneously both when using bicarbonate and CO2 as expected when using pulse labeling. Subsequent analysis of 15N enrichment in plant parts with high and low 14C activity showed that 15N also had a heterogeneous distribution (up to two orders of magnitude).ConclusionBicarbonate can efficiently be used to introduce 14C or 13C into plant via the leaf-labeling method. Both 14C and 15N showed heterogeneous distribution in the plant, although the distribution of 15N was more even than that of 14C.

AB - Aims: Application of carbon (C) and nitrogen (N) isotopes is an essential tool to study C and N flows in plant-soil-microorganisms systems. When targeting single plants in a community the tracers need to be added via e.g., leaf-labeling or stem-feeding approaches. In this study we: (i) investigated if bicarbonate can be used to introduce 14C (or 13C) into white clover and ryegrass, and (ii) compared the patterns of 14C and 15N allocation in white clover and ryegrass to evaluate the homogeneity of tracer distribution after two alternative labeling approaches.MethodsPerennial ryegrass and white clover were pulse labeled with 15N urea via leaf-labeling and 14C either via a 14CO2 atm or with 14C bicarbonate through leaf-labeling. Plants were sampled 4 days after labeling and prepared for bulk isotope analysis and for 14C imaging to identify plant parts with high and low 14C activity. Subsequently, plant parts with high and low 14C activity were separated and analyzed for 15N enrichment.ResultsBicarbonate applied by leaf-labeling efficiently introduced 14C into both white clover and ryegrass, although the 14C activity in particular for white clover was found predominantly in the labeled leaf. Using 14C imaging for identification of areas with high (hotspots) and low 14C activity showed that 14C was incorporated very heterogeneously both when using bicarbonate and CO2 as expected when using pulse labeling. Subsequent analysis of 15N enrichment in plant parts with high and low 14C activity showed that 15N also had a heterogeneous distribution (up to two orders of magnitude).ConclusionBicarbonate can efficiently be used to introduce 14C or 13C into plant via the leaf-labeling method. Both 14C and 15N showed heterogeneous distribution in the plant, although the distribution of 15N was more even than that of 14C.

U2 - 10.1007/s11104-013-1660-x

DO - 10.1007/s11104-013-1660-x

M3 - Journal article

VL - 371

SP - 191

EP - 198

JO - Plant and Soil

JF - Plant and Soil

SN - 0032-079X

IS - 1-2

ER -