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Best practice standards for circular RNA research

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  • Anne Færch Nielsen
  • Albrecht Bindereif, Justus Liebig University Giessen
  • ,
  • Irene Bozzoni, University of Rome La Sapienza
  • ,
  • Mor Hanan, Hebrew University of Jerusalem
  • ,
  • Thomas Birkballe Hansen
  • ,
  • Manuel Irimia, Barcelona Institute of Science and Technology, Pompeu Fabra University, ICREA
  • ,
  • Sebastian Kadener, Brandeis University
  • ,
  • Lasse Sommer Kristensen
  • Ivano Legnini, Helmholtz Association of German Research Centres
  • ,
  • Mariangela Morlando, University of Perugia
  • ,
  • Morten Tobias Jarlstad Olesen
  • R Jeroen Pasterkamp, Utrecht University
  • ,
  • Stephan Preibisch, Helmholtz Association of German Research Centres
  • ,
  • Nikolaus Rajewsky, Helmholtz Association of German Research Centres
  • ,
  • Christin Sünkel, Helmholtz Association of German Research Centres
  • ,
  • Jørgen Kjems

Circular RNAs (circRNAs) are formed in all domains of life and via different mechanisms. There has been an explosion in the number of circRNA papers in recent years; however, as a relatively young field, circRNA biology has an urgent need for common experimental standards for isolating, analyzing, expressing and depleting circRNAs. Here we propose a set of guidelines for circRNA studies based on the authors’ experience. This Perspective will specifically address the major class of circRNAs in Eukarya that are generated by a spliceosome-catalyzed back-splicing event. We hope that the implementation of best practice principles for circRNA research will help move the field forward and allow a better functional understanding of this fascinating group of RNAs.

Original languageEnglish
JournalNature Methods
Pages (from-to)1208-1220
Number of pages13
Publication statusPublished - Oct 2022

    Research areas

  • RNA Splicing, RNA, Circular, RNA/genetics

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