Best practice standards for circular RNA research

Anne Færch Nielsen; Albrecht Bindereif; Irene  Bozzoni; Mor Hanan; Thomas Birkballe Hansen; Manuel Irimia; Sebastian Kadener; Lasse Sommer Kristensen; Ivano Legnini; Mariangela Morlando; Morten Tobias Jarlstad Olesen; R Jeroen Pasterkamp; Stephan Preibisch; Nikolaus Rajewsky; Christin Sünkel; Jørgen Kjems

doi:10.1038/s41592-022-01487-2

Best practice standards for circular RNA research

Anne Færch Nielsen, Albrecht Bindereif, Irene Bozzoni, Mor Hanan, Thomas Birkballe Hansen, Manuel Irimia, Sebastian Kadener, Lasse Sommer Kristensen, Ivano Legnini, Mariangela Morlando, Morten Tobias Jarlstad Olesen, R Jeroen Pasterkamp, Stephan Preibisch, Nikolaus Rajewsky, Christin Sünkel, Jørgen Kjems^*

^*Corresponding author for this work

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

94 Citations (Scopus)

Abstract

Circular RNAs (circRNAs) are formed in all domains of life and via different mechanisms. There has been an explosion in the number of circRNA papers in recent years; however, as a relatively young field, circRNA biology has an urgent need for common experimental standards for isolating, analyzing, expressing and depleting circRNAs. Here we propose a set of guidelines for circRNA studies based on the authors’ experience. This Perspective will specifically address the major class of circRNAs in Eukarya that are generated by a spliceosome-catalyzed back-splicing event. We hope that the implementation of best practice principles for circRNA research will help move the field forward and allow a better functional understanding of this fascinating group of RNAs.

Original language	English
Journal	Nature Methods
Volume	19
Issue	10
Pages (from-to)	1208-1220
Number of pages	13
ISSN	1548-7091
DOIs	https://doi.org/10.1038/s41592-022-01487-2
Publication status	Published - Oct 2022

Keywords

RNA Splicing
RNA, Circular
RNA/genetics

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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9759028/pdf/nihms-1852105.pdf

Cite this

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title = "Best practice standards for circular RNA research",

abstract = "Circular RNAs (circRNAs) are formed in all domains of life and via different mechanisms. There has been an explosion in the number of circRNA papers in recent years; however, as a relatively young field, circRNA biology has an urgent need for common experimental standards for isolating, analyzing, expressing and depleting circRNAs. Here we propose a set of guidelines for circRNA studies based on the authors{\textquoteright} experience. This Perspective will specifically address the major class of circRNAs in Eukarya that are generated by a spliceosome-catalyzed back-splicing event. We hope that the implementation of best practice principles for circRNA research will help move the field forward and allow a better functional understanding of this fascinating group of RNAs.",

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doi = "10.1038/s41592-022-01487-2",

language = "English",

volume = "19",

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journal = "Nature Methods",

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T1 - Best practice standards for circular RNA research

AU - Nielsen, Anne Færch

AU - Bindereif, Albrecht

AU - Bozzoni, Irene

AU - Hanan, Mor

AU - Hansen, Thomas Birkballe

AU - Irimia, Manuel

AU - Kadener, Sebastian

AU - Kristensen, Lasse Sommer

AU - Legnini, Ivano

AU - Morlando, Mariangela

AU - Olesen, Morten Tobias Jarlstad

AU - Pasterkamp, R Jeroen

AU - Preibisch, Stephan

AU - Rajewsky, Nikolaus

AU - Sünkel, Christin

AU - Kjems, Jørgen

PY - 2022/10

Y1 - 2022/10

N2 - Circular RNAs (circRNAs) are formed in all domains of life and via different mechanisms. There has been an explosion in the number of circRNA papers in recent years; however, as a relatively young field, circRNA biology has an urgent need for common experimental standards for isolating, analyzing, expressing and depleting circRNAs. Here we propose a set of guidelines for circRNA studies based on the authors’ experience. This Perspective will specifically address the major class of circRNAs in Eukarya that are generated by a spliceosome-catalyzed back-splicing event. We hope that the implementation of best practice principles for circRNA research will help move the field forward and allow a better functional understanding of this fascinating group of RNAs.

AB - Circular RNAs (circRNAs) are formed in all domains of life and via different mechanisms. There has been an explosion in the number of circRNA papers in recent years; however, as a relatively young field, circRNA biology has an urgent need for common experimental standards for isolating, analyzing, expressing and depleting circRNAs. Here we propose a set of guidelines for circRNA studies based on the authors’ experience. This Perspective will specifically address the major class of circRNAs in Eukarya that are generated by a spliceosome-catalyzed back-splicing event. We hope that the implementation of best practice principles for circRNA research will help move the field forward and allow a better functional understanding of this fascinating group of RNAs.

KW - RNA Splicing

KW - RNA, Circular

KW - RNA/genetics

U2 - 10.1038/s41592-022-01487-2

DO - 10.1038/s41592-022-01487-2

M3 - Journal article

C2 - 35618955

SN - 1548-7091

VL - 19

SP - 1208

EP - 1220

JO - Nature Methods

JF - Nature Methods

IS - 10

ER -

Best practice standards for circular RNA research

Abstract

Keywords

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