Best practice standards for circular RNA research

Anne Færch Nielsen, Albrecht Bindereif, Irene Bozzoni, Mor Hanan, Thomas Birkballe Hansen, Manuel Irimia, Sebastian Kadener, Lasse Sommer Kristensen, Ivano Legnini, Mariangela Morlando, Morten Tobias Jarlstad Olesen, R Jeroen Pasterkamp, Stephan Preibisch, Nikolaus Rajewsky, Christin Sünkel, Jørgen Kjems*

*Corresponding author for this work

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Abstract

Circular RNAs (circRNAs) are formed in all domains of life and via different mechanisms. There has been an explosion in the number of circRNA papers in recent years; however, as a relatively young field, circRNA biology has an urgent need for common experimental standards for isolating, analyzing, expressing and depleting circRNAs. Here we propose a set of guidelines for circRNA studies based on the authors’ experience. This Perspective will specifically address the major class of circRNAs in Eukarya that are generated by a spliceosome-catalyzed back-splicing event. We hope that the implementation of best practice principles for circRNA research will help move the field forward and allow a better functional understanding of this fascinating group of RNAs.

Original languageEnglish
JournalNature Methods
Volume19
Issue10
Pages (from-to)1208-1220
Number of pages13
ISSN1548-7091
DOIs
Publication statusPublished - Oct 2022

Keywords

  • RNA Splicing
  • RNA, Circular
  • RNA/genetics

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