TY - JOUR
T1 - Basal and insulin mediated VLDL-triglyceride kinetics in type 2 diabetic men
AU - Sørensen, Lars P
AU - Andersen, Iben Rahbek
AU - Larsen, Svend Erik
AU - Søndergaard, Esben
AU - Gormsen, Lars C
AU - Schmitz, Ole
AU - Christiansen, Jens S
AU - Nielsen, Søren
PY - 2011
Y1 - 2011
N2 - AbstractObjective. Increased VLDL-TG concentration is a central feature of diabetic dyslipidemia. The objective was to compare basal and insulin mediated VLDL-TG kinetics, oxidation, and adipose tissue storage in type 2 diabetic and healthy men. Research Design and Methods. Eleven type 2 diabetic and 11 healthy men, matched for BMI and age, were included. Ex vivo-labeled VLDL-TG tracers, blood- and breath samples, fat biopsies, indirect calorimetry, and body composition measures were applied to determine VLDL-TG kinetics, VLDL-TG FA oxidation, and -storage in regional adipose tissue before and during a hyperinsulinemic euglycaemic clamp. Results. VLDL-TG secretion was significantly greater in diabetic compared with healthy men (Basal: 86.9 (31.0) vs. 61.9 (30.0) μmol/min, p=0.03; Clamp: 60.0 (26.2) vs. 34.2 (17.9) μmol·min(-1), p=0.01). The insulin mediated suppression of VLDL-TG secretion was significant in both groups. VLDL-TG clearance was lower in diabetic men (Basal: 84.6 (32.7) vs. 115.4 (44.3) ml·min(-1), p=0.08; Clamp: 76.3 (30.6) vs. 119.0 (50.2) ml·min(-1), p=0.03). During hyperinsulinemia fractional VLDL-TG FA oxidation was comparable, but in percent of EE, significantly higher in diabetic men. Basal VLDL-TG storage was similar, but significantly greater in abdominal compared with leg fat. Conclusions. Increased VLDL-TG in type 2 diabetic men is caused by greater VLDL-TG secretion and less so by lower VLDL-TG clearance. The ability of hyperinsulinemia to suppress VLDL-TG secretion appears preserved. During hyperinsulinemia VLDL-TG FA oxidation is significantly increased in proportion of EE in type 2 diabetic men. Greater basal abdominal VLDL-TG storage may help explain the accumulation of upper-body fat in insulin resistant individuals.
AB - AbstractObjective. Increased VLDL-TG concentration is a central feature of diabetic dyslipidemia. The objective was to compare basal and insulin mediated VLDL-TG kinetics, oxidation, and adipose tissue storage in type 2 diabetic and healthy men. Research Design and Methods. Eleven type 2 diabetic and 11 healthy men, matched for BMI and age, were included. Ex vivo-labeled VLDL-TG tracers, blood- and breath samples, fat biopsies, indirect calorimetry, and body composition measures were applied to determine VLDL-TG kinetics, VLDL-TG FA oxidation, and -storage in regional adipose tissue before and during a hyperinsulinemic euglycaemic clamp. Results. VLDL-TG secretion was significantly greater in diabetic compared with healthy men (Basal: 86.9 (31.0) vs. 61.9 (30.0) μmol/min, p=0.03; Clamp: 60.0 (26.2) vs. 34.2 (17.9) μmol·min(-1), p=0.01). The insulin mediated suppression of VLDL-TG secretion was significant in both groups. VLDL-TG clearance was lower in diabetic men (Basal: 84.6 (32.7) vs. 115.4 (44.3) ml·min(-1), p=0.08; Clamp: 76.3 (30.6) vs. 119.0 (50.2) ml·min(-1), p=0.03). During hyperinsulinemia fractional VLDL-TG FA oxidation was comparable, but in percent of EE, significantly higher in diabetic men. Basal VLDL-TG storage was similar, but significantly greater in abdominal compared with leg fat. Conclusions. Increased VLDL-TG in type 2 diabetic men is caused by greater VLDL-TG secretion and less so by lower VLDL-TG clearance. The ability of hyperinsulinemia to suppress VLDL-TG secretion appears preserved. During hyperinsulinemia VLDL-TG FA oxidation is significantly increased in proportion of EE in type 2 diabetic men. Greater basal abdominal VLDL-TG storage may help explain the accumulation of upper-body fat in insulin resistant individuals.
U2 - 10.2337/db10-0564
DO - 10.2337/db10-0564
M3 - Journal article
C2 - 20858686
SN - 0012-1797
VL - 60
SP - 88
EP - 96
JO - Diabetes
JF - Diabetes
IS - 1
ER -