Barley starch bioengineering for high phosphate and amylose

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  • Per Gunnar Andreas Blennow, Planteglykobiologi, Denmark
  • Massimiliano Carciofi, Denmark
  • Shahnoor Sultana Shaik, Planteglykobiologi, Denmark
  • Susanne Langgård Jensen, Molekylær Plantebiologi, Denmark
  • Jan Tommy Svensson, Planteglykobiologi, Denmark
  • Preben Bach Holm, Denmark
  • Kim Hebelstrup
Starch is a biological polymer that can be industrially produced in massive
amounts in a very pure form. Cereals is the main source for starch production
and any improvement of the starch fraction can have a tremendous impact in
food and feed applications. Barley ranks number four among cereal crops and
barley is a genetically very well characterized. Aiming at producing new
starch qualities in the cereal system, we used RNAi and overexpression
strategies to produce pure amylose and high-phosphate starch, respectively,
using the barley kernel as a polymer factory. By simultaneous silencing of the
three genes encoding the starch-branching enzymes SBEI, SBEIIa, and
SBEIIb using a triple RNAi chimeric hairpin construct we generated a
virtually amylopectin-free barley. The grains of the transgenic lines were
shrunken and had a yield of around 80% of the control line. The starch
granules were irregular and showed no distinct melting enthalpy and very
weak X-ray scattering. Hyperphosphorylated barley starch was achieved by
endosperm specific overexpression of the potato glucan water dikinase1
(StGWD1). The content of phosphate esters in this starch was tenfold higher
than the control lines. Amylose content was not affected but the starch
granules had several pores on the surfaces and a decreased melting enthalpy
indicating starch degradation stimulated by phosphate-induced amorphisation.
Our work demonstrates the feasibility of starch bioengineering to produce
completely novels starch-based polymers implementing two new strategies for
in planta starch bioengineering of cereals.
Original languageEnglish
JournalCereal Foods World
Volume56
Issue4
Pages (from-to)A15
Number of pages1
ISSN0146-6283
Publication statusPublished - 2011
Event2011 AACC International Annual Meeting - Palm Springs Convention Center, Palm Springs, California, United States
Duration: 16 Oct 201119 Oct 2011

Conference

Conference2011 AACC International Annual Meeting
LocationPalm Springs Convention Center
CountryUnited States
CityPalm Springs, California
Period16/10/201119/10/2011

Bibliographical note

Supplement to Cereal Foods World vol. 56 No 4

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