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Bacterial successions in the Broiler Gastrointestinal tract

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feeding trial was performed with broilers receiving diets based on wheat (WBF), maize (MBF), and maize-based concentrates supplemented with 15% (CKMS-15) or 30% (CKMS-30) crimped kernel maize silage. The aim of the study was to investigate the bacterial community compositions in the contents of crop, gizzard, ileum and ceca in relation to the feeding strategy and age (8, 15, 22, 25, 29 and 36 days). Of the four dietary treatments, bacterial diversity was analyzed for MBF and CKMS-30 by 454-pyrosequencing of 16S rRNA gene. Since there was no significant influence of diets on bacterial diversity, data were pooled for downstream analysis. With increasing age, a clear succession of bacterial communities and an increased bacterial diversity was observed. Lactobacillaceae (mainly Lactobacillus) represented most of the Firmicutes at all ages and in all segments of the gut except the ceca. The development of a “mature” microbiota in broilers occurred during the period from 15-22 days. A striking increase in the relative abundance of L. salivarius (17-36%) and clostridia (11-18%), and a concomitant decrease of L. reuteri was found in the ileum after d 15. De-conjugated bile salts increased in concentration in association with the increased populations of L. salivarius and clostridia. Both L. salivarius and clostridia de-conjugate bile acids, and an increase of these bacteria might be associated with growth reduction and GI disorders occurring in the critical period of broiler life between 20-30 days.
Original languageEnglish
JournalApplied and Environmental Microbiology
Volume82
Issue8
Pages (from-to)2399-2410
Number of pages11
ISSN0099-2240
DOIs
Publication statusPublished - Apr 2016

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