Bacterial interactions during sequential degradation of cyanobacterial necromass in a sulfidic arctic marine sediment

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  • Albert L. Mueller, Portland State Univ, Oregon University System, Portland State University, Dept Civil & Environm Engn
  • ,
  • Claus Pelikan, Austrian Polar Res Inst
  • ,
  • Julia R. de Rezende, Lyell Ctr Earth & Marine Sci & Technol
  • ,
  • Kenneth Wasmund, Austrian Polar Res Inst
  • ,
  • Martina Putz, Swedish Univ Agr Sci, Swedish University of Agricultural Sciences, Dept Forest Mycol & Plant Pathol
  • ,
  • Clemens Glombitza, NASA, Ames Research Center, National Aeronautics & Space Administration (NASA), Wyle Labs, Ames Res Ctr
  • ,
  • Kasper U. Kjeldsen
  • Bo Barker Jorgensen
  • Alexander Loy, Austrian Polar Res Inst

Seafloor microorganisms impact global carbon cycling by mineralizing vast quantities of organic matter (OM) from pelagic primary production, which is predicted to increase in the Arctic because of diminishing sea ice cover. We studied microbial interspecies-carbon-flow during anaerobic OM degradation in arctic marine sediment using stable isotope probing. We supplemented sediment incubations with C-13-labeled cyanobacterial necromass (spirulina), mimicking fresh OM input, or acetate, an important OM degradation intermediate and monitored sulfate reduction rates and concentrations of volatile fatty acids (VFAs) during substrate degradation. Sequential 16S rRNA gene and transcript amplicon sequencing and fluorescence in situ hybridization combined with Raman microspectroscopy revealed that only few bacterial species were the main degraders of C-13-spirulina necromass. Psychrilyobacter, Psychromonas, Marinifilum, Colwellia, Marinilabiaceae and Clostridiales species were likely involved in the primary hydrolysis and fermentation of spirulina. VFAs, mainly acetate, produced from spirulina degradation were mineralized by sulfate-reducing bacteria and an Arcobacter species. Cellular activity of Desulfobacteraceae and Desulfobulbaceae species during acetoclastic sulfate reduction was largely decoupled from relative 16S rRNA gene abundance shifts. Our findings provide new insights into the identities and physiological constraints that determine the population dynamics of key microorganisms during complex OM degradation in arctic marine sediments.(c) 2018 Society for Applied Microbiology and John Wiley & Sons Ltd

Original languageEnglish
JournalEnvironmental Microbiology
Volume20
Issue8
Pages (from-to)2927-2940
Number of pages14
ISSN1462-2912
DOIs
Publication statusPublished - Aug 2018

    Research areas

  • SULFATE-REDUCING BACTERIA, ORGANIC-MATTER, SP-NOV, MICROBIAL COMMUNITY, EXTRACELLULAR ENZYMES, CARBON DEGRADATION, ELEMENTAL SULFUR, SURFACE SEDIMENT, FJORD SEDIMENTS, SEA BED

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