TY - JOUR
T1 - Bacillus Licheniformis CotA Laccase Mutant
T2 - ElectrocatalyticReduction of O
2
from 0.6 V (SHE) at pH 8 and in Seawater
AU - Lopes, Paula
AU - Koschorreck, Katja
AU - Nedergaard Pedersen, Jannik
AU - Ferapontov, Alexey
AU - Lörcher, Samuel
AU - Skov Pedersen, Jan
AU - Urlacher, Vlada B.
AU - Ferapontova, Elena E.
PY - 2019
Y1 - 2019
N2 -
Enzymes operating in environmental media are of immense biotechnological interest, in particular, for sustainable energy production. Here, we show that the K316 N/D500G mutant of CotA laccase from Bacillus licheniformis operates in basic media and seawater, electrocatalyzing O
2
reduction from 0.59 V, pH 8. CotA mutant's T1Cu center exhibits a formal potential of 0.56 V, consistent with an onset of bioelectrocatalytic reduction of O
2
. A photoelectrochemical cell recycling H
2
O/O
2
by a semiconductor photoanode and CotA mutant biocathode produced 41 and 11 μW cm
2
, in buffer and seawater, respectively, at pH 8, (electricity production being limited solely by the photoanode performance, reaching 240 and 220 μW cm
−2
in a Zn-biobattery design), suggesting a prospective enzyme application for clean and sustainable production of electricity from seawater and oxygen.
AB -
Enzymes operating in environmental media are of immense biotechnological interest, in particular, for sustainable energy production. Here, we show that the K316 N/D500G mutant of CotA laccase from Bacillus licheniformis operates in basic media and seawater, electrocatalyzing O
2
reduction from 0.59 V, pH 8. CotA mutant's T1Cu center exhibits a formal potential of 0.56 V, consistent with an onset of bioelectrocatalytic reduction of O
2
. A photoelectrochemical cell recycling H
2
O/O
2
by a semiconductor photoanode and CotA mutant biocathode produced 41 and 11 μW cm
2
, in buffer and seawater, respectively, at pH 8, (electricity production being limited solely by the photoanode performance, reaching 240 and 220 μW cm
−2
in a Zn-biobattery design), suggesting a prospective enzyme application for clean and sustainable production of electricity from seawater and oxygen.
KW - bacterial CotA laccase
KW - bioelectrocatalysis
KW - oxygen reduction
KW - photo-biovoltaics
KW - seawater
UR - http://www.scopus.com/inward/record.url?scp=85064340199&partnerID=8YFLogxK
U2 - 10.1002/celc.201900363
DO - 10.1002/celc.201900363
M3 - Journal article
AN - SCOPUS:85064340199
SN - 2196-0216
VL - 6
SP - 2043
EP - 2049
JO - ChemElectroChem
JF - ChemElectroChem
IS - 7
ER -