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Bacillus Licheniformis CotA Laccase Mutant: ElectrocatalyticReduction of O 2 from 0.6 V (SHE) at pH 8 and in Seawater

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Bacillus Licheniformis CotA Laccase Mutant : ElectrocatalyticReduction of O 2 from 0.6 V (SHE) at pH 8 and in Seawater. / Lopes, Paula; Koschorreck, Katja; Nedergaard Pedersen, Jannik; Ferapontov, Alexey; Lörcher, Samuel; Skov Pedersen, Jan; Urlacher, Vlada B.; Ferapontova, Elena E.

In: ChemElectroChem, Vol. 6, No. 7, 2019, p. 2043-2049.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

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@article{81b7b616806e425793cf6cbb9b1074ed,
title = "Bacillus Licheniformis CotA Laccase Mutant: ElectrocatalyticReduction of O 2 from 0.6 V (SHE) at pH 8 and in Seawater",
abstract = " Enzymes operating in environmental media are of immense biotechnological interest, in particular, for sustainable energy production. Here, we show that the K316 N/D500G mutant of CotA laccase from Bacillus licheniformis operates in basic media and seawater, electrocatalyzing O 2 reduction from 0.59 V, pH 8. CotA mutant's T1Cu center exhibits a formal potential of 0.56 V, consistent with an onset of bioelectrocatalytic reduction of O 2 . A photoelectrochemical cell recycling H 2 O/O 2 by a semiconductor photoanode and CotA mutant biocathode produced 41 and 11 μW cm 2 , in buffer and seawater, respectively, at pH 8, (electricity production being limited solely by the photoanode performance, reaching 240 and 220 μW cm −2 in a Zn-biobattery design), suggesting a prospective enzyme application for clean and sustainable production of electricity from seawater and oxygen. ",
keywords = "bacterial CotA laccase, bioelectrocatalysis, oxygen reduction, photo-biovoltaics, seawater",
author = "Paula Lopes and Katja Koschorreck and {Nedergaard Pedersen}, Jannik and Alexey Ferapontov and Samuel L{\"o}rcher and {Skov Pedersen}, Jan and Urlacher, {Vlada B.} and Ferapontova, {Elena E.}",
year = "2019",
doi = "10.1002/celc.201900363",
language = "English",
volume = "6",
pages = "2043--2049",
journal = "ChemElectroChem",
issn = "2196-0216",
publisher = "Wiley - V C H Verlag GmbH & Co. KGaA",
number = "7",

}

RIS

TY - JOUR

T1 - Bacillus Licheniformis CotA Laccase Mutant

T2 - ElectrocatalyticReduction of O 2 from 0.6 V (SHE) at pH 8 and in Seawater

AU - Lopes, Paula

AU - Koschorreck, Katja

AU - Nedergaard Pedersen, Jannik

AU - Ferapontov, Alexey

AU - Lörcher, Samuel

AU - Skov Pedersen, Jan

AU - Urlacher, Vlada B.

AU - Ferapontova, Elena E.

PY - 2019

Y1 - 2019

N2 - Enzymes operating in environmental media are of immense biotechnological interest, in particular, for sustainable energy production. Here, we show that the K316 N/D500G mutant of CotA laccase from Bacillus licheniformis operates in basic media and seawater, electrocatalyzing O 2 reduction from 0.59 V, pH 8. CotA mutant's T1Cu center exhibits a formal potential of 0.56 V, consistent with an onset of bioelectrocatalytic reduction of O 2 . A photoelectrochemical cell recycling H 2 O/O 2 by a semiconductor photoanode and CotA mutant biocathode produced 41 and 11 μW cm 2 , in buffer and seawater, respectively, at pH 8, (electricity production being limited solely by the photoanode performance, reaching 240 and 220 μW cm −2 in a Zn-biobattery design), suggesting a prospective enzyme application for clean and sustainable production of electricity from seawater and oxygen.

AB - Enzymes operating in environmental media are of immense biotechnological interest, in particular, for sustainable energy production. Here, we show that the K316 N/D500G mutant of CotA laccase from Bacillus licheniformis operates in basic media and seawater, electrocatalyzing O 2 reduction from 0.59 V, pH 8. CotA mutant's T1Cu center exhibits a formal potential of 0.56 V, consistent with an onset of bioelectrocatalytic reduction of O 2 . A photoelectrochemical cell recycling H 2 O/O 2 by a semiconductor photoanode and CotA mutant biocathode produced 41 and 11 μW cm 2 , in buffer and seawater, respectively, at pH 8, (electricity production being limited solely by the photoanode performance, reaching 240 and 220 μW cm −2 in a Zn-biobattery design), suggesting a prospective enzyme application for clean and sustainable production of electricity from seawater and oxygen.

KW - bacterial CotA laccase

KW - bioelectrocatalysis

KW - oxygen reduction

KW - photo-biovoltaics

KW - seawater

UR - http://www.scopus.com/inward/record.url?scp=85064340199&partnerID=8YFLogxK

U2 - 10.1002/celc.201900363

DO - 10.1002/celc.201900363

M3 - Journal article

AN - SCOPUS:85064340199

VL - 6

SP - 2043

EP - 2049

JO - ChemElectroChem

JF - ChemElectroChem

SN - 2196-0216

IS - 7

ER -