Abstract
Carboxypeptidase II (CBPII) in brain metabolizes the neuroactive substance N-acetyl-L-aspartyl-L-glutamate (NAGG) to yield the elements of glutamate and N-acetyl-aspartate (NAA). In peripheral organs, CBPII is known as prostrate specific membrane antigen (PSMA), which presents an important target for nuclear medicine imaging in prostate cancer. Available PSMA ligands for PET imaging do not cross the blood-brain barrier, and there is scant knowledge of the neurobiology of CBPII, despite its implication in the regulation of glutamatergic neurotransmission. In this study we used the clinical PET tracer [18 F]-PSMA-1007 ([18 F]PSMA) for an autoradiographic characterization of CGPII in rat brain. Ligand binding and displacement curves indicated a single site in brain, with KD of about 0.5 nM, and Bmax ranging from 9 nM in cortex to 19 nM in white matter (corpus callosum and fimbria) and 24 nM in hypothalamus. The binding properties of [18 F]PSMA in vitro should enable its use for autoradiographic investigations of CBPII expression in animal models of human neuropsychiatric conditions.
| Original language | English |
|---|---|
| Article number | e22280 |
| Journal | Synapse |
| Volume | 77 |
| Issue | 6 |
| Number of pages | 5 |
| ISSN | 0887-4476 |
| DOIs | |
| Publication status | Published - Nov 2023 |
Keywords
- PSMA
- [ F]-PSMA-1007
- autoradiography
- brain
- carboxypeptidase II
- Antigens, Surface/chemistry
- Humans
- Rats
- Male
- Brain/diagnostic imaging
- Animals
- Glutamate Carboxypeptidase II/chemistry
- Positron-Emission Tomography/methods