Atypical Receptor Kinase RINRK1 Required for Rhizobial Infection but not Nodule Development in Lotus japonicus

Xiaolin Li, Zhiqiong Zheng, Xiangxiao Kong, Ji Xu, Liping Qiu, Jongho Sun, Dugald Elgin Reid, Haojie Jin, Stig U Andersen, Giles E D Oldroyd, Jens Stougaard, J Allan Downie, Fang Xie

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Abstract

During the legume-rhizobium symbiotic interaction, rhizobial invasion of legumes is primarily mediated by a plant-made tubular invagination called an infection thread (IT). Here, we identify a gene in Lotus japonicus encoding a leucine-rich repeat receptor-like kinase (LRR-RLK), RINRK1 (Rhizobial Infection Receptor-like Kinase1), that is induced by Nod factors (NFs) and is involved in IT formation but not nodule organogenesis. A paralog, RINRK2, plays a relatively minor role in infection. RINRK1 is required for full induction of early infection genes, including Nodule Inception (NIN), encoding an essential nodulation transcription factor. RINRK1 displayed an infection-specific expression pattern, and NIN bound to the RINRK1 promoter, inducing its expression. RINRK1 was found to be an atypical kinase localized to the plasma membrane and did not require kinase activity for rhizobial infection. We propose RINRK1 is an infection-specific RLK, which may specifically coordinate output from NF signaling or perceive an unknown signal required for rhizobial infection.

Original languageEnglish
JournalPlant Physiology
Volume181
Issue2
Pages (from-to)804-816
Number of pages13
ISSN0032-0889
DOIs
Publication statusPublished - Oct 2019

Keywords

  • BACTERIAL
  • ERN1 TRANSCRIPTION FACTOR
  • GENE
  • HOST-SPECIFICITY
  • NODULATION
  • PLANT
  • PROTEIN
  • RECOMBINANT INBRED LINES
  • SYMBIOTICALLY DEFECTIVE-MUTANTS
  • THREAD FORMATION

Fingerprint

Dive into the research topics of 'Atypical Receptor Kinase RINRK1 Required for Rhizobial Infection but not Nodule Development in Lotus japonicus'. Together they form a unique fingerprint.

Cite this