Attempts to Target Staphylococcus aureus Induced Osteomyelitis Bone Lesions in a Juvenile Pig Model by Using Radiotracers

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

  • Pia Afzelius, Aalborg University, University of Copenhagen
  • ,
  • Aage Kristian Olsen Alstrup
  • Ole Lerberg Nielsen, University of Copenhagen
  • ,
  • Karin Michaelsen Nielsen, Aalborg University, University of Copenhagen
  • ,
  • Svend Borup Jensen, Aalborg University

Background [18F]FDG Positron Emission Tomography cannot differentiate between sterile inflammation and infection. Therefore, we, aimed to develop more specific radiotracers fitted for differentiation between sterile and septic infection to improve the diagnostic accuracy. Consequently, the clinicians can refine the treatment of, for example, prosthesis-related infection. METHODS: We examined different target points; Staphylococcus aureus biofilm (68Ga-labeled DOTA-K-A9 and DOTA-GSGK-A11), bone remodeling ([18F]NaF), bacterial cell membranes ([68Ga]Ga-Ubiquicidin), and leukocyte trafficking ([68Ga]Ga-DOTA-Siglec-9). We compared them to the well-known glucose metabolism marker [18F]FDG, in a well-established juvenile S. aureus induced osteomyelitis (OM) pig model. RESULTS: [18F]FDG accumulated in the OM lesions seven days after bacterial inoculation, but disappointingly we were not able to identify any tracer accumulation in OM with any of the supposedly more specific tracers. CONCLUSION: These negative results are, however, relevant to report as they may save other research groups from conducting the same animal experiments and provide a platform for developing and evaluating other new potential tracers or protocol instead.

Original languageEnglish
Article number4329
JournalMolecules (Basel, Switzerland)
Volume25
Issue18
Number of pages15
ISSN1420-3049
DOIs
Publication statusPublished - Sep 2020

    Research areas

  • animal experimentation, osteomyelitis, PET/CT, pigs, [18F]FDG, [18F]NaF, [68Ga]Ga-DOTA-GSGK-A11 [68Ga]Ga-DOTA-Siglec-9, [68Ga]Ga-DOTA-K-A9, [68Ga]Ga-ubiquicidin

See relations at Aarhus University Citationformats

ID: 197844452