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Assessment of chemical mixtures using biomarkers of combined biological activity: A screening study in human placentas

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Assessment of chemical mixtures using biomarkers of combined biological activity : A screening study in human placentas. / Rodríguez-Carrillo, Andrea; Rosenmai, Anna Kjerstine; Mustieles, Vicente; Couderq, Stephan; Fini, Jean-Baptiste; Vela-Soria, Fernando; Molina-Molina, Jose Manuel; Ferrando-Marco, Patricia; Wielsøe, Maria; Long, Manhai; Bonefeld-Jorgensen, Eva Cecilie; Olea, Nicolás; Vinggaard, Anne Marie; Fernández, Mariana F.

In: Reproductive toxicology (Elmsford, N.Y.), Vol. 100, 03.2021, p. 143-154.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Rodríguez-Carrillo, A, Rosenmai, AK, Mustieles, V, Couderq, S, Fini, J-B, Vela-Soria, F, Molina-Molina, JM, Ferrando-Marco, P, Wielsøe, M, Long, M, Bonefeld-Jorgensen, EC, Olea, N, Vinggaard, AM & Fernández, MF 2021, 'Assessment of chemical mixtures using biomarkers of combined biological activity: A screening study in human placentas', Reproductive toxicology (Elmsford, N.Y.), vol. 100, pp. 143-154. https://doi.org/10.1016/j.reprotox.2021.01.002

APA

Rodríguez-Carrillo, A., Rosenmai, A. K., Mustieles, V., Couderq, S., Fini, J-B., Vela-Soria, F., Molina-Molina, J. M., Ferrando-Marco, P., Wielsøe, M., Long, M., Bonefeld-Jorgensen, E. C., Olea, N., Vinggaard, A. M., & Fernández, M. F. (2021). Assessment of chemical mixtures using biomarkers of combined biological activity: A screening study in human placentas. Reproductive toxicology (Elmsford, N.Y.), 100, 143-154. https://doi.org/10.1016/j.reprotox.2021.01.002

CBE

Rodríguez-Carrillo A, Rosenmai AK, Mustieles V, Couderq S, Fini J-B, Vela-Soria F, Molina-Molina JM, Ferrando-Marco P, Wielsøe M, Long M, Bonefeld-Jorgensen EC, Olea N, Vinggaard AM, Fernández MF. 2021. Assessment of chemical mixtures using biomarkers of combined biological activity: A screening study in human placentas. Reproductive toxicology (Elmsford, N.Y.). 100:143-154. https://doi.org/10.1016/j.reprotox.2021.01.002

MLA

Vancouver

Rodríguez-Carrillo A, Rosenmai AK, Mustieles V, Couderq S, Fini J-B, Vela-Soria F et al. Assessment of chemical mixtures using biomarkers of combined biological activity: A screening study in human placentas. Reproductive toxicology (Elmsford, N.Y.). 2021 Mar;100:143-154. https://doi.org/10.1016/j.reprotox.2021.01.002

Author

Rodríguez-Carrillo, Andrea ; Rosenmai, Anna Kjerstine ; Mustieles, Vicente ; Couderq, Stephan ; Fini, Jean-Baptiste ; Vela-Soria, Fernando ; Molina-Molina, Jose Manuel ; Ferrando-Marco, Patricia ; Wielsøe, Maria ; Long, Manhai ; Bonefeld-Jorgensen, Eva Cecilie ; Olea, Nicolás ; Vinggaard, Anne Marie ; Fernández, Mariana F. / Assessment of chemical mixtures using biomarkers of combined biological activity : A screening study in human placentas. In: Reproductive toxicology (Elmsford, N.Y.). 2021 ; Vol. 100. pp. 143-154.

Bibtex

@article{d25d3e48a4204eb6a7d2d2b38b64fd87,
title = "Assessment of chemical mixtures using biomarkers of combined biological activity: A screening study in human placentas",
abstract = "Humans are simultaneously exposed to complex mixtures of chemicals with limited knowledge on potential health effects, therefore improved tools for assessing these mixtures are needed. As part of the Human Biomonitoring for Europe (HBM4EU) Project, we aimed to examine the combined biological activity of chemical mixtures extracted from human placentas using one in vivo and four in vitro bioassays, also known as biomarkers of combined effect. Relevant endocrine activities (proliferative and/or reporter gene assays) and four endpoints were tested: the estrogen receptor (ER), androgen receptor (AR), and aryl hydrocarbon receptor (AhR) activities, as well as thyroid hormone (TH) signaling. Correlations among bioassays and their functional shapes were evaluated. Results showed that all placental extracts agonized or antagonized at least three of the abovementioned endpoints. Most placentas induced ER-mediated transactivation and ER-dependent cell proliferation, together with a strong inhibition of TH signaling and the AR transactivity; while the induction of the AhR was found in only one placental extract. The effects in the two estrogenic bioassays were positively and significantly correlated and the AR-antagonism activity showed a positive borderline-significant correlation with both estrogenic bioassay activities. However, the in vivo anti-thyroid activities of placental extracts were not correlated with any of the tested in vitro assays. Findings highlight the importance of comprehensively mapping the biological effects of {"}real-world{"} chemical mixtures present in human samples, through a battery of in vitro and in vivo bioassays. This approach should be a complementary tool for epidemiological studies to further elucidate the combined biological fingerprint triggered by chemical mixtures.",
keywords = "bioassay, biomarkers, chemical mixtures, combined effect, endocrine disruption, HBM4EU, placenta, reproduction",
author = "Andrea Rodr{\'i}guez-Carrillo and Rosenmai, {Anna Kjerstine} and Vicente Mustieles and Stephan Couderq and Jean-Baptiste Fini and Fernando Vela-Soria and Molina-Molina, {Jose Manuel} and Patricia Ferrando-Marco and Maria Wiels{\o}e and Manhai Long and Bonefeld-Jorgensen, {Eva Cecilie} and Nicol{\'a}s Olea and Vinggaard, {Anne Marie} and Fern{\'a}ndez, {Mariana F}",
note = "Copyright {\textcopyright} 2021. Published by Elsevier Inc.",
year = "2021",
month = mar,
doi = "10.1016/j.reprotox.2021.01.002",
language = "English",
volume = "100",
pages = "143--154",
journal = "Reproductive Toxicology",
issn = "0890-6238",
publisher = "Elsevier Inc.",

}

RIS

TY - JOUR

T1 - Assessment of chemical mixtures using biomarkers of combined biological activity

T2 - A screening study in human placentas

AU - Rodríguez-Carrillo, Andrea

AU - Rosenmai, Anna Kjerstine

AU - Mustieles, Vicente

AU - Couderq, Stephan

AU - Fini, Jean-Baptiste

AU - Vela-Soria, Fernando

AU - Molina-Molina, Jose Manuel

AU - Ferrando-Marco, Patricia

AU - Wielsøe, Maria

AU - Long, Manhai

AU - Bonefeld-Jorgensen, Eva Cecilie

AU - Olea, Nicolás

AU - Vinggaard, Anne Marie

AU - Fernández, Mariana F

N1 - Copyright © 2021. Published by Elsevier Inc.

PY - 2021/3

Y1 - 2021/3

N2 - Humans are simultaneously exposed to complex mixtures of chemicals with limited knowledge on potential health effects, therefore improved tools for assessing these mixtures are needed. As part of the Human Biomonitoring for Europe (HBM4EU) Project, we aimed to examine the combined biological activity of chemical mixtures extracted from human placentas using one in vivo and four in vitro bioassays, also known as biomarkers of combined effect. Relevant endocrine activities (proliferative and/or reporter gene assays) and four endpoints were tested: the estrogen receptor (ER), androgen receptor (AR), and aryl hydrocarbon receptor (AhR) activities, as well as thyroid hormone (TH) signaling. Correlations among bioassays and their functional shapes were evaluated. Results showed that all placental extracts agonized or antagonized at least three of the abovementioned endpoints. Most placentas induced ER-mediated transactivation and ER-dependent cell proliferation, together with a strong inhibition of TH signaling and the AR transactivity; while the induction of the AhR was found in only one placental extract. The effects in the two estrogenic bioassays were positively and significantly correlated and the AR-antagonism activity showed a positive borderline-significant correlation with both estrogenic bioassay activities. However, the in vivo anti-thyroid activities of placental extracts were not correlated with any of the tested in vitro assays. Findings highlight the importance of comprehensively mapping the biological effects of "real-world" chemical mixtures present in human samples, through a battery of in vitro and in vivo bioassays. This approach should be a complementary tool for epidemiological studies to further elucidate the combined biological fingerprint triggered by chemical mixtures.

AB - Humans are simultaneously exposed to complex mixtures of chemicals with limited knowledge on potential health effects, therefore improved tools for assessing these mixtures are needed. As part of the Human Biomonitoring for Europe (HBM4EU) Project, we aimed to examine the combined biological activity of chemical mixtures extracted from human placentas using one in vivo and four in vitro bioassays, also known as biomarkers of combined effect. Relevant endocrine activities (proliferative and/or reporter gene assays) and four endpoints were tested: the estrogen receptor (ER), androgen receptor (AR), and aryl hydrocarbon receptor (AhR) activities, as well as thyroid hormone (TH) signaling. Correlations among bioassays and their functional shapes were evaluated. Results showed that all placental extracts agonized or antagonized at least three of the abovementioned endpoints. Most placentas induced ER-mediated transactivation and ER-dependent cell proliferation, together with a strong inhibition of TH signaling and the AR transactivity; while the induction of the AhR was found in only one placental extract. The effects in the two estrogenic bioassays were positively and significantly correlated and the AR-antagonism activity showed a positive borderline-significant correlation with both estrogenic bioassay activities. However, the in vivo anti-thyroid activities of placental extracts were not correlated with any of the tested in vitro assays. Findings highlight the importance of comprehensively mapping the biological effects of "real-world" chemical mixtures present in human samples, through a battery of in vitro and in vivo bioassays. This approach should be a complementary tool for epidemiological studies to further elucidate the combined biological fingerprint triggered by chemical mixtures.

KW - bioassay

KW - biomarkers

KW - chemical mixtures

KW - combined effect

KW - endocrine disruption

KW - HBM4EU

KW - placenta

KW - reproduction

U2 - 10.1016/j.reprotox.2021.01.002

DO - 10.1016/j.reprotox.2021.01.002

M3 - Journal article

C2 - 33444715

VL - 100

SP - 143

EP - 154

JO - Reproductive Toxicology

JF - Reproductive Toxicology

SN - 0890-6238

ER -