Assays to Monitor Autophagy Progression in Cell Cultures

Idil Orhon; Fulvio Reggiori

doi:10.3390/cells6030020

Assays to Monitor Autophagy Progression in Cell Cultures

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Abstract

The vast number of implications of autophagy in multiple areas of life sciences and medicine has attracted the interest of numerous scientists that aim to unveil the role of this process in specific physiological and pathological contexts. Cell cultures are one of the most frequently used experimental setup for the investigation of autophagy. As a result, it is essential to assess this highly regulated molecular pathway with efficient and reliable methods. Each method has its own advantages and disadvantages. Here, we present a review summarizing the most established assays used to monitor autophagy induction and progression in cell cultures, in order to guide researchers in the selection of the most optimal solution for their experimental setup and design.

Original language	English
Journal	Cells
Volume	6
Issue	3
ISSN	2073-4409
DOIs	https://doi.org/10.3390/cells6030020
Publication status	Published - 7 Jul 2017

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abstract = "The vast number of implications of autophagy in multiple areas of life sciences and medicine has attracted the interest of numerous scientists that aim to unveil the role of this process in specific physiological and pathological contexts. Cell cultures are one of the most frequently used experimental setup for the investigation of autophagy. As a result, it is essential to assess this highly regulated molecular pathway with efficient and reliable methods. Each method has its own advantages and disadvantages. Here, we present a review summarizing the most established assays used to monitor autophagy induction and progression in cell cultures, in order to guide researchers in the selection of the most optimal solution for their experimental setup and design.",

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Assays to Monitor Autophagy Progression in Cell Cultures

Abstract

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