Artificial extracellular matrix delivers TGFb1 regulating myofibroblast differentiation

Weilu Cheng; Ruodan Xu; Dalong Li; Christian Bortolini; Jinmei He; MD Dong; Flemming Besenbacher; Yudong Huang; Menglin Chen

doi:10.1039/c5ra26164c

Artificial extracellular matrix delivers TGFb1 regulating myofibroblast differentiation

Weilu Cheng, Ruodan Xu, Dalong Li, Christian Bortolini, Jinmei He, MD Dong, Flemming Besenbacher, Yudong Huang, Menglin Chen

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

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Abstract

During wound healing, the contractile activity of myofibroblasts differentiated from fibroblasts in active transforming growth factor β1 (TGFβ1) conditions has vital functions. In wound dressing biomaterials, it is crucial to mimic the extracellular matrix to deliver the right amount of TGFβ1 in a spatiotemporally controlled manner. We report here, for the first time, a zero-order, sustained TGFβ1 release from electrospun biomimetic nanofibers realizing optimal cell viability and myofibroblast differentiation capacity, confirmed by cell metabolic activity CCK assay, gene expression level through real-time PCR and protein expression level through immunochemical staining.

Original language	English
Journal	RSC Advances
Volume	6
Issue	26
Pages (from-to)	21922-21928
Number of pages	7
ISSN	2046-2069
DOIs	https://doi.org/10.1039/c5ra26164c
Publication status	Published - 2016

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title = "Artificial extracellular matrix delivers TGFb1 regulating myofibroblast differentiation",

abstract = "During wound healing, the contractile activity of myofibroblasts differentiated from fibroblasts in active transforming growth factor β1 (TGFβ1) conditions has vital functions. In wound dressing biomaterials, it is crucial to mimic the extracellular matrix to deliver the right amount of TGFβ1 in a spatiotemporally controlled manner. We report here, for the first time, a zero-order, sustained TGFβ1 release from electrospun biomimetic nanofibers realizing optimal cell viability and myofibroblast differentiation capacity, confirmed by cell metabolic activity CCK assay, gene expression level through real-time PCR and protein expression level through immunochemical staining.",

author = "Weilu Cheng and Ruodan Xu and Dalong Li and Christian Bortolini and Jinmei He and MD Dong and Flemming Besenbacher and Yudong Huang and Menglin Chen",

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T1 - Artificial extracellular matrix delivers TGFb1 regulating myofibroblast differentiation

AU - Cheng, Weilu

AU - Xu, Ruodan

AU - Li, Dalong

AU - Bortolini, Christian

AU - He, Jinmei

AU - Dong, MD

AU - Besenbacher, Flemming

AU - Huang, Yudong

AU - Chen, Menglin

PY - 2016

Y1 - 2016

N2 - During wound healing, the contractile activity of myofibroblasts differentiated from fibroblasts in active transforming growth factor β1 (TGFβ1) conditions has vital functions. In wound dressing biomaterials, it is crucial to mimic the extracellular matrix to deliver the right amount of TGFβ1 in a spatiotemporally controlled manner. We report here, for the first time, a zero-order, sustained TGFβ1 release from electrospun biomimetic nanofibers realizing optimal cell viability and myofibroblast differentiation capacity, confirmed by cell metabolic activity CCK assay, gene expression level through real-time PCR and protein expression level through immunochemical staining.

AB - During wound healing, the contractile activity of myofibroblasts differentiated from fibroblasts in active transforming growth factor β1 (TGFβ1) conditions has vital functions. In wound dressing biomaterials, it is crucial to mimic the extracellular matrix to deliver the right amount of TGFβ1 in a spatiotemporally controlled manner. We report here, for the first time, a zero-order, sustained TGFβ1 release from electrospun biomimetic nanofibers realizing optimal cell viability and myofibroblast differentiation capacity, confirmed by cell metabolic activity CCK assay, gene expression level through real-time PCR and protein expression level through immunochemical staining.

U2 - 10.1039/c5ra26164c

DO - 10.1039/c5ra26164c

M3 - Journal article

SN - 2046-2069

VL - 6

SP - 21922

EP - 21928

JO - RSC Advances

JF - RSC Advances

IS - 26

ER -

Artificial extracellular matrix delivers TGFb1 regulating myofibroblast differentiation

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