Arabidopsis pollen tube integrity and sperm release are regulated by RALF-mediated signaling

Zengxiang Ge; Tabata Bergonci; Yuling Zhao; Yanjiao Zou; Shuo Du; Ming Che Liu; Xingju Luo; Hao Ruan; Liliana E. García-Valencia; Sheng Zhong; Saiying Hou; Qingpei Huang; Luhua Lai; Daniel S. Moura; Hongya Gu; Juan Dong; Hen Ming Wu; Thomas Dresselhaus; Junyu Xiao; Alice Y. Cheung; Li Jia Qu

doi:10.1126/science.aao3642

Arabidopsis pollen tube integrity and sperm release are regulated by RALF-mediated signaling

Zengxiang Ge, Tabata Bergonci, Yuling Zhao, Yanjiao Zou, Shuo Du, Ming Che Liu, Xingju Luo, Hao Ruan, Liliana E. García-Valencia, Sheng Zhong, Saiying Hou, Qingpei Huang, Luhua Lai, Daniel S. Moura, Hongya Gu, Juan Dong, Hen Ming Wu, Thomas Dresselhaus, Junyu Xiao, Alice Y. CheungLi Jia Qu^*

^*Corresponding author for this work

Department of Forensic Medicine - Retskemisk

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

299 Citations (Scopus)

Abstract

In flowering plants, fertilization requires complex cell-to-cell communication events between the pollen tube and the female reproductive tissues, which are controlled by extracellular signaling molecules interacting with receptors at the pollen tube surface.We found that two such receptors in Arabidopsis, BUPS1 and BUPS2, and their peptide ligands, RALF4 and RALF19, are pollen tube-expressed and are required to maintain pollen tube integrity. BUPS1 and BUPS2 interact with receptors ANXUR1 and ANXUR2 via their ectodomains, and both sets of receptors bind RALF4 and RALF19. These receptor-ligand interactions are in competition with the female-derived ligand RALF34, which induces pollen tube bursting at nanomolar concentrations.We propose that RALF34 replaces RALF4 and RALF19 at the interface of pollen tube-female gametophyte contact, thereby deregulating BUPS-ANXUR signaling and in turn leading to pollen tube rupture and sperm release.

Original language	English
Journal	Science
Volume	358
Issue	6370
Pages (from-to)	1596-1600
Number of pages	5
ISSN	0036-8075
DOIs	https://doi.org/10.1126/science.aao3642
Publication status	Published - 22 Dec 2017

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Ge, Z., Bergonci, T., Zhao, Y., Zou, Y., Du, S., Liu, M. C., Luo, X., Ruan, H., García-Valencia, L. E., Zhong, S., Hou, S., Huang, Q., Lai, L., Moura, D. S., Gu, H., Dong, J., Wu, H. M., Dresselhaus, T., Xiao, J., ... Qu, L. J. (2017). Arabidopsis pollen tube integrity and sperm release are regulated by RALF-mediated signaling. Science, 358(6370), 1596-1600. https://doi.org/10.1126/science.aao3642

@article{24b56d3db5004516956ab274af16804d,

title = "Arabidopsis pollen tube integrity and sperm release are regulated by RALF-mediated signaling",

abstract = "In flowering plants, fertilization requires complex cell-to-cell communication events between the pollen tube and the female reproductive tissues, which are controlled by extracellular signaling molecules interacting with receptors at the pollen tube surface.We found that two such receptors in Arabidopsis, BUPS1 and BUPS2, and their peptide ligands, RALF4 and RALF19, are pollen tube-expressed and are required to maintain pollen tube integrity. BUPS1 and BUPS2 interact with receptors ANXUR1 and ANXUR2 via their ectodomains, and both sets of receptors bind RALF4 and RALF19. These receptor-ligand interactions are in competition with the female-derived ligand RALF34, which induces pollen tube bursting at nanomolar concentrations.We propose that RALF34 replaces RALF4 and RALF19 at the interface of pollen tube-female gametophyte contact, thereby deregulating BUPS-ANXUR signaling and in turn leading to pollen tube rupture and sperm release.",

author = "Zengxiang Ge and Tabata Bergonci and Yuling Zhao and Yanjiao Zou and Shuo Du and Liu, {Ming Che} and Xingju Luo and Hao Ruan and Garc{\'i}a-Valencia, {Liliana E.} and Sheng Zhong and Saiying Hou and Qingpei Huang and Luhua Lai and Moura, {Daniel S.} and Hongya Gu and Juan Dong and Wu, {Hen Ming} and Thomas Dresselhaus and Junyu Xiao and Cheung, {Alice Y.} and Qu, {Li Jia}",

year = "2017",

month = dec,

day = "22",

doi = "10.1126/science.aao3642",

language = "English",

volume = "358",

pages = "1596--1600",

journal = "Science",

issn = "0036-8075",

publisher = "American Association for the Advancement of Science",

number = "6370",

}

Ge, Z, Bergonci, T, Zhao, Y, Zou, Y, Du, S, Liu, MC, Luo, X, Ruan, H, García-Valencia, LE, Zhong, S, Hou, S, Huang, Q, Lai, L, Moura, DS, Gu, H, Dong, J, Wu, HM, Dresselhaus, T, Xiao, J, Cheung, AY & Qu, LJ 2017, 'Arabidopsis pollen tube integrity and sperm release are regulated by RALF-mediated signaling', Science, vol. 358, no. 6370, pp. 1596-1600. https://doi.org/10.1126/science.aao3642

TY - JOUR

T1 - Arabidopsis pollen tube integrity and sperm release are regulated by RALF-mediated signaling

AU - Ge, Zengxiang

AU - Bergonci, Tabata

AU - Zhao, Yuling

AU - Zou, Yanjiao

AU - Du, Shuo

AU - Liu, Ming Che

AU - Luo, Xingju

AU - Ruan, Hao

AU - García-Valencia, Liliana E.

AU - Zhong, Sheng

AU - Hou, Saiying

AU - Huang, Qingpei

AU - Lai, Luhua

AU - Moura, Daniel S.

AU - Gu, Hongya

AU - Dong, Juan

AU - Wu, Hen Ming

AU - Dresselhaus, Thomas

AU - Xiao, Junyu

AU - Cheung, Alice Y.

AU - Qu, Li Jia

PY - 2017/12/22

Y1 - 2017/12/22

N2 - In flowering plants, fertilization requires complex cell-to-cell communication events between the pollen tube and the female reproductive tissues, which are controlled by extracellular signaling molecules interacting with receptors at the pollen tube surface.We found that two such receptors in Arabidopsis, BUPS1 and BUPS2, and their peptide ligands, RALF4 and RALF19, are pollen tube-expressed and are required to maintain pollen tube integrity. BUPS1 and BUPS2 interact with receptors ANXUR1 and ANXUR2 via their ectodomains, and both sets of receptors bind RALF4 and RALF19. These receptor-ligand interactions are in competition with the female-derived ligand RALF34, which induces pollen tube bursting at nanomolar concentrations.We propose that RALF34 replaces RALF4 and RALF19 at the interface of pollen tube-female gametophyte contact, thereby deregulating BUPS-ANXUR signaling and in turn leading to pollen tube rupture and sperm release.

AB - In flowering plants, fertilization requires complex cell-to-cell communication events between the pollen tube and the female reproductive tissues, which are controlled by extracellular signaling molecules interacting with receptors at the pollen tube surface.We found that two such receptors in Arabidopsis, BUPS1 and BUPS2, and their peptide ligands, RALF4 and RALF19, are pollen tube-expressed and are required to maintain pollen tube integrity. BUPS1 and BUPS2 interact with receptors ANXUR1 and ANXUR2 via their ectodomains, and both sets of receptors bind RALF4 and RALF19. These receptor-ligand interactions are in competition with the female-derived ligand RALF34, which induces pollen tube bursting at nanomolar concentrations.We propose that RALF34 replaces RALF4 and RALF19 at the interface of pollen tube-female gametophyte contact, thereby deregulating BUPS-ANXUR signaling and in turn leading to pollen tube rupture and sperm release.

UR - http://www.scopus.com/inward/record.url?scp=85038381934&partnerID=8YFLogxK

U2 - 10.1126/science.aao3642

DO - 10.1126/science.aao3642

M3 - Journal article

C2 - 29242234

AN - SCOPUS:85038381934

SN - 0036-8075

VL - 358

SP - 1596

EP - 1600

JO - Science

JF - Science

IS - 6370

ER -

Arabidopsis pollen tube integrity and sperm release are regulated by RALF-mediated signaling

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