Aptamer-Targeted Drug Delivery for Staphylococcus aureus Biofilm

Pernille Ommen, Line Hansen, Bente K Hansen, Hieu Vu-Quang, Jørgen Kjems, Rikke L Meyer*

*Corresponding author for this work

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Abstract

Treatment of Staphylococcus aureus biofilm infections using conventional antibiotic therapy is challenging as only doses that are sublethal to the biofilm can be administered safely to patients. A potential solution to this challenge is targeted drug delivery. In this study, we tailored an aptamer-targeted liposomal drug delivery system for accumulation and delivery of antibiotics locally in S. aureus biofilm. In our search for a suitable targeting ligand, we identified six DNA aptamers that bound to S. aureus cells in biofilms, and we demonstrated that one of these aptamers could facilitate accumulation of liposomes around S. aureus cells inside the biofilm. Aptamer-targeted liposomes encapsulating a combination of vancomycin and rifampicin were able to eradicate S. aureus biofilm upon 24 h of treatment in vitro. Our results point to that aptamer-targeted drug delivery of antibiotics is a potential new strategy for treatment of S. aureus biofilm infections.

Original languageEnglish
Article number814340
JournalFrontiers in cellular and infection microbiology
Volume12
Number of pages12
ISSN2235-2988
DOIs
Publication statusPublished - Apr 2022

Keywords

  • Anti-Bacterial Agents/therapeutic use
  • Biofilms
  • Drug Delivery Systems
  • Humans
  • Liposomes
  • Microbial Sensitivity Tests
  • Staphylococcal Infections/drug therapy
  • Staphylococcus aureus/genetics

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