Abstract
The objective of the present explorative study was to determine the absorption dynamics when feeding diets varying in types and levels of
dietary fibre in a catheterised animal model. A total of six sows were fed a diet low in fibre (LF), a diet high in soluble fibre and a diet high
in insoluble fibre in a repeated 3 £ 3 cross-over design. Plasma samples were collected from the mesenteric artery and the portal vein to
determine different absorption phases by 1H NMR spectroscopy-based metabonomics. Time profiles were determined for plasma levels of
specific metabolites and for the absorption of these metabolites from the small intestine. The LF diet resulted in a higher betaine concentration
in the blood than the two high-fibre diets (P¼0·008). This leads to higher plasma concentrations of methionine (P¼0·0028) and
creatine (P¼0·020) of endogenous origin. In conclusion, the use of NMR spectroscopy for measuring nutrient uptake in the present
study elucidated the relationship between betaine uptake and elevated creatine plasma concentrations.
dietary fibre in a catheterised animal model. A total of six sows were fed a diet low in fibre (LF), a diet high in soluble fibre and a diet high
in insoluble fibre in a repeated 3 £ 3 cross-over design. Plasma samples were collected from the mesenteric artery and the portal vein to
determine different absorption phases by 1H NMR spectroscopy-based metabonomics. Time profiles were determined for plasma levels of
specific metabolites and for the absorption of these metabolites from the small intestine. The LF diet resulted in a higher betaine concentration
in the blood than the two high-fibre diets (P¼0·008). This leads to higher plasma concentrations of methionine (P¼0·0028) and
creatine (P¼0·020) of endogenous origin. In conclusion, the use of NMR spectroscopy for measuring nutrient uptake in the present
study elucidated the relationship between betaine uptake and elevated creatine plasma concentrations.
| Original language | English |
|---|---|
| Journal | British Journal of Nutrition |
| Volume | 107 |
| Issue | 11 |
| Pages (from-to) | 1603-1615 |
| Number of pages | 13 |
| ISSN | 0007-1145 |
| DOIs | |
| Publication status | Published - Jun 2012 |