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Anti-tumor activity of a B-cell receptor-targeted peptide in a novel disseminated lymphoma xenograft model

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

  • Claudia Wehr
  • ,
  • Fabian Müller
  • ,
  • Julia Schüler
  • ,
  • Tina Tomann
  • ,
  • Claudia Nitschke
  • ,
  • Henning Seismann
  • ,
  • Edzard Spillner
  • Kerstin Klingner
  • ,
  • Tanja Schneider-Merck
  • ,
  • Mascha Binder
  • ,
  • Heinz-Herbert Fiebig
  • ,
  • Roland Mertelsmann
  • ,
  • Martin Trepel
Receptor-targeted therapies have become standard in the treatment of various lymphomas. In view of its unparalleled specificity for the malignant B-cell clone, the B-cell receptor (BCR) on B cell lymphoma cells is a potential therapeutic target. We have used two BCR epitope mimicking peptides binding to the Burkitt's lymphoma cell lines CA46 and SUP-B8. We proved their functionality by demonstrating calcium flux and BCR-mediated endocytosis upon peptide receptor binding. Toxicity experiments in vitro via cross-linking of the BCR with tetramerized epitope mimics lead to apoptosis in both cell lines but was far more effective in SUP-B8 cells. We established a SUP-B8-based disseminated Burkitt's lymphoma model in NOD/SCID mice. Treatment of tumor-bearing mice with tetramerized epitope mimics had significant anti-tumor effects in vivo. We conclude that peptide-mediated, BCR-targeted therapy is a promising approach which may be explored and further developed for application in highly aggressive lymphoma.
Original languageEnglish
JournalInternational Journal of Cancer
Pages (from-to)E10-20
Publication statusPublished - 15 Jul 2012
Externally publishedYes

    Research areas

  • Animals, Apoptosis, Burkitt Lymphoma, Calcium, Cell Line, Tumor, Endocytosis, Epitopes, B-Lymphocyte, Female, Humans, Mice, Mice, Inbred NOD, Mice, SCID, Molecular Mimicry, Molecular Targeted Therapy, Peptides, Receptors, Antigen, B-Cell, Xenograft Model Antitumor Assays

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