Antibacterial and immunomodulatory effects of Pheromonicin-NM on Escherichia coli-challenged bovine mammary epithelial cells

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  • Guiyan Yang, China Agricultural University
  • ,
  • Yuan Yue
  • ,
  • Dan Li, China Agricultural University
  • ,
  • Cong Duan, China Agricultural University
  • ,
  • Xiaoqing Qiu, Sichuan University
  • ,
  • Yunjing Zou, China Agricultural University
  • ,
  • Yaohong Zhu, China Agricultural University
  • ,
  • Charlotte Lauridsen
  • Jiufeng Wang, China Agricultural University

Mastitis affects cows in all regions of the world and Escherichia coli (E. coli) is by far the most common reason of mastitis. Now antibiotic therapy is still the preferred approach of treating mastitis. However, antibiotic usage is easy to lead to antibiotic resistance. There is an urgent need for developing efficacious alternative antimicrobials. Pheromonicin-NM (PMC-NM) is a new engineered bactericidal peptide consisting of colicin Ia and an anti-porin A antibody mimetic. It can lead to the dissipation of cellular energy and therefore kill the bacteria rapidly. The aim of the present study was to investigate the comparative effects of PMC-NM and antibiotic ceftiofur on antibacterial and innate immune responses of bovine mammary epithelial cells (BMEC) to E. coli infection. We found that E. coli growth was inhibited by PMC-NM from 0.5 h after treatment and was completely inhibited at 3 h, indicating a rapid antibacterial activity for PMC-NM. The mRNA expression of TLR2, IL-1β, IL-8, lactoferrin, LAP, TAP and DEFB1 was increased by PMC-NM treatment at 2 h after E. coli infection, suggesting the enhanced inflammatory responses induced by PMC-NM contribute to pathogens clearance at early phase. By contrast, in E. coli-infected BMECs, ceftiofur treatment upregulated TLR2 and NOD2 levels at 12 h, and extremely elevated transcription levels of TNF-α, IL-1β, IL-8, lactoferrin, LAP, TAP, BNBD5, DEFB1 at 6 h. The excessive expression of these genes at later phase can induce uncontrolled inflammatory responses and finally cause damage. Taken together, PMC-NM might be used as an ideal antibacterial agent against E. coli mastitis.

Original languageEnglish
Article number106569
JournalInternational Immunopharmacology
Volume84
ISSN1567-5769
DOIs
Publication statusPublished - Jul 2020

    Research areas

  • Bovine mammary epithelial cells, Ceftiofur, Escherichia coli, Pathogen recognition receptors, Pheromonicin-NM

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