Animal Models Used to Simulate Retinal Artery Occlusion: A Comprehensive Review

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperReviewResearchpeer-review

DOI

  • Nanna Vestergaard, Department of Ophthalmology, Aalborg University Hospital, Aalborg, Denmark; Department of Clinical Medicine, Aalborg University, Aalborg, Denmark.
  • ,
  • Lasse Jørgensen Cehofski, Department of Endocrinology, Odense University Hospital, Odense, Denmark ; Department of Gynecology and Obstetrics, Odense University Hospital, Odense, Denmark ; Institute of Clinical Research, University of Southern Denmark, Odense, Denmark.
  • ,
  • Bent Honoré
  • Kristian Aasbjerg, Department of Clinical Epidemiology, Institute of Clinical Medicine, Aarhus University Hospital, Aalborg, Denmark Department of Cardiology, Aalborg University Hospital, Aalborg, Denmark Department of Anaesthesia and Intensive Care Medicine, Aalborg University Hospital, Aalborg, Denmark Aalborg Atrial Fibrillation Study Group, Aalborg, Denmark.
  • ,
  • Henrik Vorum

Purpose: To present an overview of animal models of retinal artery occlusion (RAO).

Methods: Through a systematic literature search in PubMed and Embase, papers describing methods of inducing RAO in animal models were included. The identified methodologic approaches were presented in a narrative synthesis and compared with RAO in humans.

Results: In total, 83 papers reporting on 88 experiments were included. Six different species were used with rodents and monkeys being the most common, and a minority were performed using cats, dogs, rabbits, or pigs. The anatomy of pigs and monkeys resemble that of humans most closely. The two most frequently used methods were laser-induced occlusion or ligation of the arteries. Other methods included raised intraocular pressure, arterial clamping, administration of vasoconstricting agents, the use of an occluder, embolization, and endovascular approaches to induce occlusion. In general, occlusions lasted for only 30 to 90 minutes, often followed by reperfusion.

Conclusions: Although a broad range of methods have previously been used, they all have limitations. Preferably, the methods should imitate the human disease as closely as possible and avoid damaging other structures. Therefore, monkeys followed by pigs are to be preferred and ligation or clamping may be a suitable model in larger animals as there is a potential to isolate and occlude the retinal artery only. Being less invasive, laser-induced occlusion is another suitable approach.

Translational Relevance: This review aims at assisting researchers in deciding on the most ideal experimental setting, and thereby increase the translational value to human disease.

Original languageEnglish
Article number23
JournalTranslational vision science & technology
Volume8
Issue4
Number of pages14
ISSN2164-2591
DOIs
Publication statusPublished - 2019

    Research areas

  • CHOROIDAL BLOOD-FLOW, CYNOMOLGUS MONKEY, HYPERBARIC-OXYGEN, INTRAOCULAR-PRESSURE, ISCHEMIA-REPERFUSION INJURY, LASER-DOPPLER FLOWMETRY, OPTIC-NERVE HEAD, RAT MODEL, TRANSIENT COMPLETE OBSTRUCTION, VASCULAR OCCLUSION, animal models, experimental models, in vivo models, retinal artery occlusion, retinal ischemia

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