Analytical Evaluation of Low-Field 31P NMR Spectroscopy for Lipid Analysis

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We investigate the potential of P-31 NMR with simple, maintenance-free benchtop spectrometers to probe phospholipids in complex mixtures. P-31 NMR-based lipidomics has become an important topic in a wide range of applications in food- and health-sciences, and the continuous improvements of compact, maintenance- and cryogen-free instruments opens new opportunities for NMR routine analyses. A prior milestone is the evaluation of the analytical performance provided by P-31 NMR at low magnetic field. To address this, we assess the ability of state-of-the-art benchtop NMR spectrometers to detect, identify, and quantify several types of phospholipids in mixtures. Relying on heteronuclear cross-polarization experiments, phospholipids can be detected in 2 h with a limit of detection of 0.5 mM at 1 T and 0.2 mM at 2 T, while the headgroups of phosphatidylcholine (PC), phosphatidyl-ethanolamine (PE), phosphatidylinositol (PI), phosphatidylserine (PS), and phosphatidyl-glycerol (PG) can be unambiguously assigned based on 2D H-1-P-31 total correlated spectroscopy (TOCSY) spectra. Furthermore, two quantitative methods to obtain absolute concentrations are proposed and discussed, and the performance is evaluated regarding precision and accuracy.

Original languageEnglish
JournalAnalytical Chemistry
Volume91
Issue4
Pages (from-to)3035-3042
Number of pages8
ISSN0003-2700
DOIs
Publication statusPublished - Feb 2019

    Research areas

  • AUTHENTICATION, CROSS-POLARIZATION, H-1-NMR SPECTROSCOPY, NUCLEAR-MAGNETIC-RESONANCE, PHOSPHOLIPIDS, VIRGIN OLIVE OIL

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