Analysis of t(9;17)(q33.2;q25.3) chromosomal breakpoint regions and genetic association reveals novel candidate genes for bipolar disorder

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  • A.P. Rajkumar, Denmark
  • Jane H. Christensen
  • Manuel Mattheisen
  • Iben Jacobsen, Translational Neuropsychiatry Unit, Department of Clinical Medicine, Aarhus University, Denmark., Denmark
  • Iben Bache, Wilhelm Johannsen Centre for Functional Genome Research, Department of Cellular and Molecular Medicine, Faculty of Health Science, University of Copenhagen, Denmark
  • Jonatan Pallesen
  • Jakob Grove
  • Per Qvist
  • Andrew Mcquillin, Molecular Psychiatry Laboratory, Division of Psychiatry, University College London, United Kingdom
  • Hugh M. Gurling, Molecular Psychiatry Laboratory, Division of Psychiatry, University College London, United Kingdom
  • Zeynep Tümer, Applied Molecular Genetics, Kennedy Center, Copenhagen University Hospital, Rigshospitalet, Denmark
  • Ole Mors
  • Anders D. Børglum

Objectives: Breakpoints of chromosomal abnormalities facilitate identification of novel candidate genes for psychiatric disorders. Genome-wide significant evidence supports the linkage between chromosome 17q25.3 and bipolar disorder (BD). Co-segregation of translocation t(9;17)(q33.2;q25.3) with psychiatric disorders has been reported. We aimed to narrow down these chromosomal breakpoint regions and to investigate the associations between single nucleotide polymorphisms within these regions and BD as well as schizophrenia (SZ) in large genome-wide association study samples. Methods: We cross-linked Danish psychiatric and cytogenetic case registers to identify an individual with both t(9;17)(q33.2;q25.3) and BD. Fluorescent in situ hybridization was employed to map the chromosomal breakpoint regions of this proband. We accessed the Psychiatric Genomics Consortium BD (n = 16,731) and SZ (n = 21,856) data. Genetic associations between these disorders and single nucleotide polymorphisms within these breakpoint regions were analysed by BioQ, FORGE, and RegulomeDB programmes. Results: Four protein-coding genes [coding for (endonuclease V (ENDOV), neuronal pentraxin I (NPTX1), ring finger protein 213 (RNF213), and regulatory-associated protein of mammalian target of rapamycin (mTOR) (RPTOR)] were found to be located within the 17q25.3 breakpoint region. NPTX1 was significantly associated with BD (p = 0.004), while ENDOV was significantly associated with SZ (p = 0.0075) after Bonferroni correction. Conclusions: Prior linkage evidence and our findings suggest NPTX1 as a novel candidate gene for BD.

Original languageEnglish
JournalBipolar Disorders (English Edition, Online)
Volume17
Issue2
Pages (from-to)205-211
Number of pages7
ISSN1398-5647
DOIs
Publication statusPublished - 1 Jan 2015

    Research areas

  • Bipolar disorder, Genetic association studies, Genetic translocation, NPTX1, RPTOR

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