An update to polyketide synthase and non-ribosomal synthetase genes and nomenclature in Fusarium

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  • Frederik T Hansen, Denmark
  • Donald M Gardiner, Commonwealth Scientific and Industrial Research Organisation (CSIRO) Agriculture Flagship, Queensland Bioscience Precinct, Brisbane, Queensland, Australia, Australia
  • Erik Lysøe, Department of Plant Health and Plant Protection, Bioforsk–Norwegian Institute of Agricultural and Environmental Research, Norway
  • Patricia Romans Fuertes, Aalborg University, Denmark
  • Bettina Tudzynski, Institut für Biologie und Biotechnologie der Pflanzen, Molecular Biology and Biotechnology of Fungi, Westfälische Wilhelms-Universität Münster, Germany
  • Philipp Wiemann, Department of Medical Microbiology and Immunology, University of Wisconsin, Madison, United States
  • Teis Esben Sondergaard, Aalborg University, Denmark
  • Henriette Giese, Institut for Kemi og Biovidenskab, Denmark
  • Ditlev E. Brodersen
  • Jens Laurids Sørensen, Aalborg University, Denmark

Members of the genus Fusarium produce a plethora of bioactive secondary metabolites, which can be harmful to humans and animals or have potential in drug development. In this study we have performed comparative analyses of polyketide synthases (PKSs) and non-ribosomal peptide synthetases (NRPSs) from ten different Fusarium species including F. graminearum (two strains), F. verticillioides, F. solani, F. culmorum, F. pseudograminearum, F. fujikuroi, F. acuminatum, F. avenaceum, F. equiseti, and F. oxysporum (12 strains). This led to identification of 52 NRPS and 52 PKSs orthology groups, respectively, and although not all PKSs and NRPSs are assumed to be intact or functional, the analyses illustrate the huge secondary metabolite potential in Fusarium. In our analyses we identified a core collection of eight NRPSs (NRPS2-4, 6, 10-13) and two PKSs (PKS3 and PKS7) that are conserved in all strains analyzed in this study. The identified PKSs and NRPSs were named based on a previously developed classification system (www.FusariumNRPSPKS.dk). We suggest this system be used when PKSs and NRPSs have to be classified in future sequenced Fusarium strains. This system will facilitate identification of orthologous and non-orthologous NRPSs and PKSs from newly sequenced Fusarium genomes and will aid the scientific community by providing a common nomenclature for these two groups of genes/enzymes.

Original languageEnglish
JournalFungal Genetics and Biology
Volume75
Pages (from-to)20-29
Number of pages10
ISSN1087-1845
DOIs
Publication statusPublished - Feb 2015

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