An RNA origami octahedron with intrinsic siRNAs for potent gene knockdown

Hans Christian Høiberg; Steffen Møller Sparvath; Veronica Liv Andersen; Jørgen Kjems; Ebbe Sloth Andersen

doi:10.1002/biot.201700634

An RNA origami octahedron with intrinsic siRNAs for potent gene knockdown

Hans Christian Høiberg, Steffen Møller Sparvath, Veronica Liv Andersen, Jørgen Kjems, Ebbe Sloth Andersen

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

Abstract

The fields of DNA and RNA nanotechnology have established nucleic acids as valuable building blocks for functional nanodevices with applications in nanomedicine. Here, a simple method for designing and assembling a 3D scaffolded RNA origami wireframe structure with intrinsic functioning small interfering RNAs (siRNAs) embedded is introduced. Uniquely, the method uses an mRNA fragment as scaffold strand, which is folded by sequence-complementarity of nine shorter synthetic strands. High-yield production of the intended 3D structure is verified by transmission electron microscopy (TEM). Production of functional siRNAs is facilitated by incorporating recognition sites for Dicer at selected locations in the structure, and efficient silencing of a target reporter gene is demonstrated.

Original language	English
Article number	1700634
Journal	Biotechnology Journal
Volume	14
Issue	1
ISSN	1860-6768
DOIs	https://doi.org/10.1002/biot.201700634
Publication status	Published - Jan 2019

Keywords

RNA design
RNA interference
RNA nanotechnology
RNA origami
self-assembly

Access to Document

10.1002/biot.201700634

Cite this

@article{c89ca5d69d104c3588519f585c0045c5,

title = "An RNA origami octahedron with intrinsic siRNAs for potent gene knockdown",

abstract = "The fields of DNA and RNA nanotechnology have established nucleic acids as valuable building blocks for functional nanodevices with applications in nanomedicine. Here, a simple method for designing and assembling a 3D scaffolded RNA origami wireframe structure with intrinsic functioning small interfering RNAs (siRNAs) embedded is introduced. Uniquely, the method uses an mRNA fragment as scaffold strand, which is folded by sequence-complementarity of nine shorter synthetic strands. High-yield production of the intended 3D structure is verified by transmission electron microscopy (TEM). Production of functional siRNAs is facilitated by incorporating recognition sites for Dicer at selected locations in the structure, and efficient silencing of a target reporter gene is demonstrated.",

keywords = "RNA design, RNA interference, RNA nanotechnology, RNA origami, self-assembly",

author = "H{\o}iberg, {Hans Christian} and Sparvath, {Steffen M{\o}ller} and Andersen, {Veronica Liv} and J{\o}rgen Kjems and Andersen, {Ebbe Sloth}",

year = "2019",

month = jan,

doi = "10.1002/biot.201700634",

language = "English",

volume = "14",

journal = "Biotechnology Journal",

issn = "1860-6768",

publisher = "Wiley - V C H Verlag GmbH & Co. KGaA",

number = "1",

}

An RNA origami octahedron with intrinsic siRNAs for potent gene knockdown. / Høiberg, Hans Christian; Sparvath, Steffen Møller; Andersen, Veronica Liv et al.
In: Biotechnology Journal, Vol. 14, No. 1, 1700634, 01.2019.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

TY - JOUR

T1 - An RNA origami octahedron with intrinsic siRNAs for potent gene knockdown

AU - Høiberg, Hans Christian

AU - Sparvath, Steffen Møller

AU - Andersen, Veronica Liv

AU - Kjems, Jørgen

AU - Andersen, Ebbe Sloth

PY - 2019/1

Y1 - 2019/1

N2 - The fields of DNA and RNA nanotechnology have established nucleic acids as valuable building blocks for functional nanodevices with applications in nanomedicine. Here, a simple method for designing and assembling a 3D scaffolded RNA origami wireframe structure with intrinsic functioning small interfering RNAs (siRNAs) embedded is introduced. Uniquely, the method uses an mRNA fragment as scaffold strand, which is folded by sequence-complementarity of nine shorter synthetic strands. High-yield production of the intended 3D structure is verified by transmission electron microscopy (TEM). Production of functional siRNAs is facilitated by incorporating recognition sites for Dicer at selected locations in the structure, and efficient silencing of a target reporter gene is demonstrated.

AB - The fields of DNA and RNA nanotechnology have established nucleic acids as valuable building blocks for functional nanodevices with applications in nanomedicine. Here, a simple method for designing and assembling a 3D scaffolded RNA origami wireframe structure with intrinsic functioning small interfering RNAs (siRNAs) embedded is introduced. Uniquely, the method uses an mRNA fragment as scaffold strand, which is folded by sequence-complementarity of nine shorter synthetic strands. High-yield production of the intended 3D structure is verified by transmission electron microscopy (TEM). Production of functional siRNAs is facilitated by incorporating recognition sites for Dicer at selected locations in the structure, and efficient silencing of a target reporter gene is demonstrated.

KW - RNA design

KW - RNA interference

KW - RNA nanotechnology

KW - RNA origami

KW - self-assembly

UR - http://www.scopus.com/inward/record.url?scp=85057280978&partnerID=8YFLogxK

U2 - 10.1002/biot.201700634

DO - 10.1002/biot.201700634

M3 - Journal article

C2 - 29802763

SN - 1860-6768

VL - 14

JO - Biotechnology Journal

JF - Biotechnology Journal

IS - 1

M1 - 1700634

ER -

An RNA origami octahedron with intrinsic siRNAs for potent gene knockdown

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this