An RNA aptamer-based electrochemical biosensor for detection of theophylline in serum

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  • Interdisciplinary Nanoscience Center
  • Department of Chemistry
An electrochemical RNA aptamer-based biosensor for rapid and label-free detection of the bronchodilator theophylline was developed. The 5'-disulfide-functionalized end of the RNA aptamer sequence was immobilized on a gold electrode, and the 3'-amino-functionalized end was conjugated with a ferrocene (Fc) redox probe. Upon binding of theophylline the aptamer switches conformation from an open unfolded state to a closed hairpin-type conformation, resulting in the increased electron-transfer efficiency between Fc and the electrode. The electrochemical response, which was measured by differential pulse voltammetry, reaches saturation within a few minutes after addition of theophylline, and the dynamic range for detecting theophylline is 0.2-10 muM. The electrode displays an inhibited response when applied directly in serum samples treated with RNase inhibitors; however a full response to the theophylline serum concentration was obtained by transferring the electrode to blank serum-free buffer solutions. It was demonstrated that theophylline is detected with high selectivity in the presence of caffeine and theobromine.
Original languageEnglish
JournalJournal of the American Chemical Society
Volume130
Issue13
Pages (from-to)4256-4258
Number of pages2
ISSN0002-7863
DOIs
Publication statusPublished - 2 Apr 2008

    Research areas

  • Aptamers, Nucleotide, Base Sequence, Biosensing Techniques, Electrochemistry, Electrodes, Gold, Humans, Sensitivity and Specificity, Surface Properties, Theophylline

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