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An inverse association between plasma benzoxazinoid metabolites and PSA after rye intake in men with prostate cancer revealed with a new method

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  • Elise Nordin, Chalmers University of Technology
  • ,
  • Stine K. Steffensen
  • ,
  • Bente B. Laursen
  • Sven Olof Andersson, Örebro University
  • ,
  • Jan Erik Johansson, Örebro University
  • ,
  • Per Åman, Swedish University of Agricultural Sciences
  • ,
  • Göran Hallmans, Umeå University
  • ,
  • Michael Borre
  • Dan Stærk, University of Copenhagen
  • ,
  • Kati Hanhineva, Chalmers University of Technology, Food Chemistry and Food Development Unit, University of Eastern Finland
  • ,
  • Inge S. Fomsgaard
  • Rikard Landberg, Chalmers University of Technology, Umeå University

Prostate cancer (PC) is a common cancer among men, and preventive strategies are warranted. Benzoxazinoids (BXs) in rye have shown potential against PC in vitro but human studies are lacking. The aim was to establish a quantitative method for analysis of BXs and investigate their plasma levels after a whole grain/bran rye vs refined wheat intervention, as well as exploring their association with PSA, in men with PC. A quantitative method for analysis of 22 BXs, including novel metabolites identified by mass spectrometry and NMR, was established, and applied to plasma samples from a randomized crossover study where patients with indolent PC (n = 17) consumed 485 g whole grain rye/rye bran or fiber supplemented refined wheat daily for 6 wk. Most BXs were significantly higher in plasma after rye (0.3–19.4 nmol/L in plasma) vs. refined wheat (0.05–2.9 nmol/L) intake. HBOA-glc, 2-HHPAA, HBOA-glcA, 2-HPAA-glcA were inversely correlated to PSA in plasma (p < 0.04). To conclude, BXs in plasma, including metabolites not previously analyzed, were quantified. BX metabolites were significantly higher after rye vs refined wheat consumption. Four BX-related metabolites were inversely associated with PSA, which merits further investigation.

Original languageEnglish
Article number5260
JournalScientific Reports
Number of pages14
Publication statusPublished - 28 Mar 2022

Bibliographical note

© 2022. The Author(s).

    Research areas

  • Benzoxazines/metabolism, Cross-Over Studies, Humans, Male, Prostate-Specific Antigen/metabolism, Prostatic Neoplasms, Secale/metabolism

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