Amplification-free liquid biopsy by fluorescence approach

Jesper Uhd; Anders Okholm; Jargen Kjems; Kira Astakhova

Amplification-free liquid biopsy by fluorescence approach

Jesper Uhd, Anders Okholm, Jargen Kjems, Kira Astakhova

Interdisciplinary Nanoscience Center - INANO-MBG, iNANO-huset

Research output: Contribution to book/anthology/report/proceeding › Article in proceedings › Research › peer-review

Abstract

Liquid biopsy is an attractive new paradigm of modern cancer research and clinical oncology. Synergy of fluorescence microscopy with mutation specific molecular probes is a method that we developed for the detection of tumor related circulating DNA, ctDNA. The present detection methods of ctDNA samples include amplification-based techniques that have multiple challenges, are often time consuming and rather expensive. In this work, we successfully applied the hybridization assay and advanced microscopy for the reliable amplification-free detection and quantification of cancer associated mutations in ctDNA.

Original language	English
Title of host publication	Biotech, Biomaterials and Biomedical - TechConnect Briefs 2017
Number of pages	4
Volume	3
Publisher	TechConnect
Publication date	1 Jan 2017
Pages	106-109
ISBN (Electronic)	9780998878201
Publication status	Published - 1 Jan 2017
Event	11th Annual TechConnect World Innovation Conference and Expo, Held Jointly with the 20th Annual Nanotech Conference and Expo, and the 2017 National SBIR/STTR Conference - Washington, United States Duration: 14 May 2017 → 17 May 2017

Conference

Conference	11th Annual TechConnect World Innovation Conference and Expo, Held Jointly with the 20th Annual Nanotech Conference and Expo, and the 2017 National SBIR/STTR Conference
Country/Territory	United States
City	Washington
Period	14/05/2017 → 17/05/2017

Keywords

Bioconjugation
CtDNA
Fluorescence microscopy
Mutation detection
Nucleic acid

Access to Document

https://briefs.techconnect.org/wp-content/volumes/TCB2017v3/pdf/762.pdf

Cite this

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title = "Amplification-free liquid biopsy by fluorescence approach",

abstract = "Liquid biopsy is an attractive new paradigm of modern cancer research and clinical oncology. Synergy of fluorescence microscopy with mutation specific molecular probes is a method that we developed for the detection of tumor related circulating DNA, ctDNA. The present detection methods of ctDNA samples include amplification-based techniques that have multiple challenges, are often time consuming and rather expensive. In this work, we successfully applied the hybridization assay and advanced microscopy for the reliable amplification-free detection and quantification of cancer associated mutations in ctDNA.",

keywords = "Bioconjugation, CtDNA, Fluorescence microscopy, Mutation detection, Nucleic acid",

author = "Jesper Uhd and Anders Okholm and Jargen Kjems and Kira Astakhova",

year = "2017",

month = jan,

day = "1",

language = "English",

volume = "3",

pages = "106--109",

booktitle = "Biotech, Biomaterials and Biomedical - TechConnect Briefs 2017",

publisher = "TechConnect",

note = "11th Annual TechConnect World Innovation Conference and Expo, Held Jointly with the 20th Annual Nanotech Conference and Expo, and the 2017 National SBIR/STTR Conference ; Conference date: 14-05-2017 Through 17-05-2017",

}

Uhd, J, Okholm, A, Kjems, J & Astakhova, K 2017, Amplification-free liquid biopsy by fluorescence approach. in Biotech, Biomaterials and Biomedical - TechConnect Briefs 2017. vol. 3, TechConnect, pp. 106-109, 11th Annual TechConnect World Innovation Conference and Expo, Held Jointly with the 20th Annual Nanotech Conference and Expo, and the 2017 National SBIR/STTR Conference, Washington, United States, 14/05/2017. <https://briefs.techconnect.org/wp-content/volumes/TCB2017v3/pdf/762.pdf>

TY - GEN

T1 - Amplification-free liquid biopsy by fluorescence approach

AU - Uhd, Jesper

AU - Okholm, Anders

AU - Kjems, Jargen

AU - Astakhova, Kira

PY - 2017/1/1

Y1 - 2017/1/1

N2 - Liquid biopsy is an attractive new paradigm of modern cancer research and clinical oncology. Synergy of fluorescence microscopy with mutation specific molecular probes is a method that we developed for the detection of tumor related circulating DNA, ctDNA. The present detection methods of ctDNA samples include amplification-based techniques that have multiple challenges, are often time consuming and rather expensive. In this work, we successfully applied the hybridization assay and advanced microscopy for the reliable amplification-free detection and quantification of cancer associated mutations in ctDNA.

AB - Liquid biopsy is an attractive new paradigm of modern cancer research and clinical oncology. Synergy of fluorescence microscopy with mutation specific molecular probes is a method that we developed for the detection of tumor related circulating DNA, ctDNA. The present detection methods of ctDNA samples include amplification-based techniques that have multiple challenges, are often time consuming and rather expensive. In this work, we successfully applied the hybridization assay and advanced microscopy for the reliable amplification-free detection and quantification of cancer associated mutations in ctDNA.

KW - Bioconjugation

KW - CtDNA

KW - Fluorescence microscopy

KW - Mutation detection

KW - Nucleic acid

UR - http://www.scopus.com/inward/record.url?scp=85029396926&partnerID=8YFLogxK

M3 - Article in proceedings

AN - SCOPUS:85029396926

VL - 3

SP - 106

EP - 109

BT - Biotech, Biomaterials and Biomedical - TechConnect Briefs 2017

PB - TechConnect

T2 - 11th Annual TechConnect World Innovation Conference and Expo, Held Jointly with the 20th Annual Nanotech Conference and Expo, and the 2017 National SBIR/STTR Conference

Y2 - 14 May 2017 through 17 May 2017

ER -

Amplification-free liquid biopsy by fluorescence approach

Abstract

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Keywords

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