Altered lead(II)-cleavage pattern of free Phe-tRNA(Phe) and Phe-tRNA(Phe) in ternary complex with EF-Tu:GTP

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Standard

Altered lead(II)-cleavage pattern of free Phe-tRNA(Phe) and Phe-tRNA(Phe) in ternary complex with EF-Tu:GTP. / Otzen, D. E.; Barciszewski, J.; Clark, B. F.C.

In: Biochemistry and Molecular Biology International, Vol. 31, No. 1, 01.01.1993, p. 95-103.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Otzen, DE, Barciszewski, J & Clark, BFC 1993, 'Altered lead(II)-cleavage pattern of free Phe-tRNA(Phe) and Phe-tRNA(Phe) in ternary complex with EF-Tu:GTP', Biochemistry and Molecular Biology International, vol. 31, no. 1, pp. 95-103.

APA

Otzen, D. E., Barciszewski, J., & Clark, B. F. C. (1993). Altered lead(II)-cleavage pattern of free Phe-tRNA(Phe) and Phe-tRNA(Phe) in ternary complex with EF-Tu:GTP. Biochemistry and Molecular Biology International, 31(1), 95-103.

CBE

Otzen DE, Barciszewski J, Clark BFC. 1993. Altered lead(II)-cleavage pattern of free Phe-tRNA(Phe) and Phe-tRNA(Phe) in ternary complex with EF-Tu:GTP. Biochemistry and Molecular Biology International. 31(1):95-103.

MLA

Otzen, D. E., J. Barciszewski and B. F.C. Clark. "Altered lead(II)-cleavage pattern of free Phe-tRNA(Phe) and Phe-tRNA(Phe) in ternary complex with EF-Tu:GTP". Biochemistry and Molecular Biology International. 1993, 31(1). 95-103.

Vancouver

Otzen DE, Barciszewski J, Clark BFC. Altered lead(II)-cleavage pattern of free Phe-tRNA(Phe) and Phe-tRNA(Phe) in ternary complex with EF-Tu:GTP. Biochemistry and Molecular Biology International. 1993 Jan 1;31(1):95-103.

Author

Otzen, D. E. ; Barciszewski, J. ; Clark, B. F.C. / Altered lead(II)-cleavage pattern of free Phe-tRNA(Phe) and Phe-tRNA(Phe) in ternary complex with EF-Tu:GTP. In: Biochemistry and Molecular Biology International. 1993 ; Vol. 31, No. 1. pp. 95-103.

Bibtex

@article{023bf8b124424a2a9043ca852db2cc49,
title = "Altered lead(II)-cleavage pattern of free Phe-tRNA(Phe) and Phe-tRNA(Phe) in ternary complex with EF-Tu:GTP",
abstract = "Pb2+ ions in sub-millimolar concentrations are known to cleave internucleotide bonds of phenylalanine-specific transfer RNA (tRNA(Phe)) from Saccharomyces. cerevisiae specifically between nucleotides D17 and G18 in the D-loop, with additional minor cleavages after D16 and G15. This makes lead(II) a sensitive structural probe for correct folding of tRNA(Phe). In the present paper we use Pb2+ ions as a functional probe to determine whether this part of tRNA is protected by the Escherichia coli elongation factor EF-Tu in the ternary complex formed between Phe-tRNA(Phe) and EF-Tu.GTP. Our results show that for tRNA in complex with EF-Tu:GTP, the phosphodiester bond after D17 is cleaved, yet the phosphodiester bonds after D16 and G15 are not. To our knowledge, this is the first time that Pb2+ ions, bound at a specific site in tRNA, have been used both to investigate the correct folding of tRNA in complex, and to footprint a functional complex with components whose individual structures are known.",
author = "Otzen, {D. E.} and J. Barciszewski and Clark, {B. F.C.}",
year = "1993",
month = jan,
day = "1",
language = "English",
volume = "31",
pages = "95--103",
journal = "Biochemistry international",
issn = "0158-5231",
publisher = "Academic Press Inc.",
number = "1",

}

RIS

TY - JOUR

T1 - Altered lead(II)-cleavage pattern of free Phe-tRNA(Phe) and Phe-tRNA(Phe) in ternary complex with EF-Tu:GTP

AU - Otzen, D. E.

AU - Barciszewski, J.

AU - Clark, B. F.C.

PY - 1993/1/1

Y1 - 1993/1/1

N2 - Pb2+ ions in sub-millimolar concentrations are known to cleave internucleotide bonds of phenylalanine-specific transfer RNA (tRNA(Phe)) from Saccharomyces. cerevisiae specifically between nucleotides D17 and G18 in the D-loop, with additional minor cleavages after D16 and G15. This makes lead(II) a sensitive structural probe for correct folding of tRNA(Phe). In the present paper we use Pb2+ ions as a functional probe to determine whether this part of tRNA is protected by the Escherichia coli elongation factor EF-Tu in the ternary complex formed between Phe-tRNA(Phe) and EF-Tu.GTP. Our results show that for tRNA in complex with EF-Tu:GTP, the phosphodiester bond after D17 is cleaved, yet the phosphodiester bonds after D16 and G15 are not. To our knowledge, this is the first time that Pb2+ ions, bound at a specific site in tRNA, have been used both to investigate the correct folding of tRNA in complex, and to footprint a functional complex with components whose individual structures are known.

AB - Pb2+ ions in sub-millimolar concentrations are known to cleave internucleotide bonds of phenylalanine-specific transfer RNA (tRNA(Phe)) from Saccharomyces. cerevisiae specifically between nucleotides D17 and G18 in the D-loop, with additional minor cleavages after D16 and G15. This makes lead(II) a sensitive structural probe for correct folding of tRNA(Phe). In the present paper we use Pb2+ ions as a functional probe to determine whether this part of tRNA is protected by the Escherichia coli elongation factor EF-Tu in the ternary complex formed between Phe-tRNA(Phe) and EF-Tu.GTP. Our results show that for tRNA in complex with EF-Tu:GTP, the phosphodiester bond after D17 is cleaved, yet the phosphodiester bonds after D16 and G15 are not. To our knowledge, this is the first time that Pb2+ ions, bound at a specific site in tRNA, have been used both to investigate the correct folding of tRNA in complex, and to footprint a functional complex with components whose individual structures are known.

UR - http://www.scopus.com/inward/record.url?scp=0027520018&partnerID=8YFLogxK

M3 - Journal article

C2 - 8260950

AN - SCOPUS:0027520018

VL - 31

SP - 95

EP - 103

JO - Biochemistry international

JF - Biochemistry international

SN - 0158-5231

IS - 1

ER -