Alterations in Blood Monocyte Functions in Parkinson's Disease

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Alterations in Blood Monocyte Functions in Parkinson's Disease. / Nissen, Sara Konstantin; Shrivastava, Kalpana; Schulte, Claudia; Otzen, Daniel Erik; Goldeck, David; Berg, Daniela; Møller, Holger Jon; Maetzler, Walter; Romero-Ramos, Marina.

In: Movement Disorders, 26.08.2019.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

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Nissen SK, Shrivastava K, Schulte C, Otzen DE, Goldeck D, Berg D et al. Alterations in Blood Monocyte Functions in Parkinson's Disease. Movement Disorders. 2019 Aug 26. https://doi.org/10.1002/mds.27815

Author

Nissen, Sara Konstantin ; Shrivastava, Kalpana ; Schulte, Claudia ; Otzen, Daniel Erik ; Goldeck, David ; Berg, Daniela ; Møller, Holger Jon ; Maetzler, Walter ; Romero-Ramos, Marina. / Alterations in Blood Monocyte Functions in Parkinson's Disease. In: Movement Disorders. 2019.

Bibtex

@article{8f9f9d00091f4b17970d4a6315715405,
title = "Alterations in Blood Monocyte Functions in Parkinson's Disease",
abstract = "BACKGROUND: PD is a multisystem disease where both central and peripheral nervous systems are affected. This systemic involvement also includes the immune response in PD, which implicates not only microglia in the brain, but also peripheral immune cells, such as monocytes; however, this aspect has been understudied.OBJECTIVES: The purpose of this study was to investigate the PD-related changes in peripheral immune cells, their responsiveness to stimulation, and their ability to release immunomodulatory molecules that might have consequences for the disease progression.METHODS: Using flow cytometry, we investigated the monocytic population in peripheral blood mononuclear cells from PD patients and healthy individuals. We also evaluated the in vitro response to inflammogen lipopolysaccharides and to fibrillar α-synuclein by measuring the expression of CD14, CD163, and HLA-DR and by analysis of soluble immune-related molecules in the supernatant.RESULTS: Peripheral blood immune cells from PD patients had lower survival in culture, but showed a higher monocytic proliferative ability than control cells, which was correlated with shorter disease duration and late disease onset. In addition, PD patients' cells were less responsive to stimulation, as shown by the lack of changes in CD163 and CD14 expression, and by the absence of significant upregulation of anti-inflammatory cytokines in culture. Moreover, PD peripheral immune cells shed lower in vitro levels of soluble CD163, which suggests a less responsive monocytic population and/or an activation status different from control cells. Interestingly, some of the results were sex associated, supporting a differential immune response in females versus males.CONCLUSIONS: Our data suggest that PD involves monocytic changes in blood. These cells show reduced viability and are unresponsive to specific stimuli, which might have a relevant consequence for disease progression. {\circledC} 2019 International Parkinson and Movement Disorder Society.",
author = "Nissen, {Sara Konstantin} and Kalpana Shrivastava and Claudia Schulte and Otzen, {Daniel Erik} and David Goldeck and Daniela Berg and M{\o}ller, {Holger Jon} and Walter Maetzler and Marina Romero-Ramos",
note = "{\circledC} 2019 International Parkinson and Movement Disorder Society.",
year = "2019",
month = "8",
day = "26",
doi = "10.1002/mds.27815",
language = "English",
journal = "Movement Disorders",
issn = "0885-3185",
publisher = "JohnWiley & Sons, Inc.",

}

RIS

TY - JOUR

T1 - Alterations in Blood Monocyte Functions in Parkinson's Disease

AU - Nissen, Sara Konstantin

AU - Shrivastava, Kalpana

AU - Schulte, Claudia

AU - Otzen, Daniel Erik

AU - Goldeck, David

AU - Berg, Daniela

AU - Møller, Holger Jon

AU - Maetzler, Walter

AU - Romero-Ramos, Marina

N1 - © 2019 International Parkinson and Movement Disorder Society.

PY - 2019/8/26

Y1 - 2019/8/26

N2 - BACKGROUND: PD is a multisystem disease where both central and peripheral nervous systems are affected. This systemic involvement also includes the immune response in PD, which implicates not only microglia in the brain, but also peripheral immune cells, such as monocytes; however, this aspect has been understudied.OBJECTIVES: The purpose of this study was to investigate the PD-related changes in peripheral immune cells, their responsiveness to stimulation, and their ability to release immunomodulatory molecules that might have consequences for the disease progression.METHODS: Using flow cytometry, we investigated the monocytic population in peripheral blood mononuclear cells from PD patients and healthy individuals. We also evaluated the in vitro response to inflammogen lipopolysaccharides and to fibrillar α-synuclein by measuring the expression of CD14, CD163, and HLA-DR and by analysis of soluble immune-related molecules in the supernatant.RESULTS: Peripheral blood immune cells from PD patients had lower survival in culture, but showed a higher monocytic proliferative ability than control cells, which was correlated with shorter disease duration and late disease onset. In addition, PD patients' cells were less responsive to stimulation, as shown by the lack of changes in CD163 and CD14 expression, and by the absence of significant upregulation of anti-inflammatory cytokines in culture. Moreover, PD peripheral immune cells shed lower in vitro levels of soluble CD163, which suggests a less responsive monocytic population and/or an activation status different from control cells. Interestingly, some of the results were sex associated, supporting a differential immune response in females versus males.CONCLUSIONS: Our data suggest that PD involves monocytic changes in blood. These cells show reduced viability and are unresponsive to specific stimuli, which might have a relevant consequence for disease progression. © 2019 International Parkinson and Movement Disorder Society.

AB - BACKGROUND: PD is a multisystem disease where both central and peripheral nervous systems are affected. This systemic involvement also includes the immune response in PD, which implicates not only microglia in the brain, but also peripheral immune cells, such as monocytes; however, this aspect has been understudied.OBJECTIVES: The purpose of this study was to investigate the PD-related changes in peripheral immune cells, their responsiveness to stimulation, and their ability to release immunomodulatory molecules that might have consequences for the disease progression.METHODS: Using flow cytometry, we investigated the monocytic population in peripheral blood mononuclear cells from PD patients and healthy individuals. We also evaluated the in vitro response to inflammogen lipopolysaccharides and to fibrillar α-synuclein by measuring the expression of CD14, CD163, and HLA-DR and by analysis of soluble immune-related molecules in the supernatant.RESULTS: Peripheral blood immune cells from PD patients had lower survival in culture, but showed a higher monocytic proliferative ability than control cells, which was correlated with shorter disease duration and late disease onset. In addition, PD patients' cells were less responsive to stimulation, as shown by the lack of changes in CD163 and CD14 expression, and by the absence of significant upregulation of anti-inflammatory cytokines in culture. Moreover, PD peripheral immune cells shed lower in vitro levels of soluble CD163, which suggests a less responsive monocytic population and/or an activation status different from control cells. Interestingly, some of the results were sex associated, supporting a differential immune response in females versus males.CONCLUSIONS: Our data suggest that PD involves monocytic changes in blood. These cells show reduced viability and are unresponsive to specific stimuli, which might have a relevant consequence for disease progression. © 2019 International Parkinson and Movement Disorder Society.

U2 - 10.1002/mds.27815

DO - 10.1002/mds.27815

M3 - Journal article

JO - Movement Disorders

JF - Movement Disorders

SN - 0885-3185

ER -