Alkaline pH increases protein extraction yield and solubility of the extracted protein from sugar kelp (Saccharina latissima)

Louise Juul, Sanne Koch Haue, Annette Bruhn, Teis Boderskov, Trine Kastrup Dalsgaard*

*Corresponding author for this work

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

7 Citations (Scopus)
241 Downloads (Pure)

Abstract

Seaweed is gaining interest as a sustainable source of protein. An often-used method for protein extraction from seaweed is alkaline extraction. In this study, the robustness of alkaline protein extraction was tested against protein extraction at neutral pH for sugar kelp (S. latissima) harvested at different time points and at different cultivation sites. The harvest time and place significantly influenced the total nitrogen (N) content of the biomass, the N extraction yield as % of biomass N, and the protein solubility. However, there was no specific pattern regarding harvest season and biomass age. The N extraction yield as % of biomass dry matter content was more similar between biomasses. The N extraction yield (% of biomass N) varied from 1.59 % to 25.22 % (mean: 6.61 %) for the control extraction, whereas it was 5.32–52.96 % (mean: 16.03 %) for the alkaline extraction. Hence, the alkaline extraction increased the N yield, and gave a higher N concentration in the protein extracts and improved the solubility of the protein extracts across the different biomass batches compared to the control method at neutral pH. This states the robustness of the alkaline protein extraction for sugar kelp and suggests improved suitability in foods compared to the extraction at neutral pH.

Original languageEnglish
JournalFood and Bioproducts Processing
Volume140
Pages (from-to)144-150
ISSN0960-3085
DOIs
Publication statusPublished - Aug 2023

Keywords

  • Alkaline protein extraction
  • Alternative food protein
  • Macroalgae
  • pH-shift
  • Protein solubility
  • Seaweed

Fingerprint

Dive into the research topics of 'Alkaline pH increases protein extraction yield and solubility of the extracted protein from sugar kelp (Saccharina latissima)'. Together they form a unique fingerprint.

Cite this