Acute changes in the metabolome following resistance exercise combined with intake of different protein sources (cricket, pea, whey)

Sofie Kaas Lanng; Mikkel Oxfeldt; Frank Ted Johansen; Jeyanthini Risikesan; Mette Hansen; Hanne Christine Bertram

doi:10.1007/s11306-023-02064-0

Acute changes in the metabolome following resistance exercise combined with intake of different protein sources (cricket, pea, whey)

Sofie Kaas Lanng, Mikkel Oxfeldt, Frank Ted Johansen, Jeyanthini Risikesan, Mette Hansen, Hanne Christine Bertram^*

^*Corresponding author for this work

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

1 Citation (Scopus)

Abstract

INTRODUCTION: Separately, both exercise and protein ingestion have been shown to alter the blood and urine metabolome. This study goes a step further and examines changes in the metabolome derived from blood, urine and muscle tissue extracts in response to resistance exercise combined with ingestion of three different protein sources.

METHODS: In an acute parallel study, 52 young males performed one-legged resistance exercise (leg extension, 4 × 10 repetitions at 10 repetition maximum) followed by ingestion of either cricket (insect), pea or whey protein (0.25 g protein/kg fat free mass). Blood and muscle tissue were collected at baseline and three hours after protein ingestion. Urine was collected at baseline and four hours after protein ingestion. Mixed-effects analyses were applied to examine the effect of the time (baseline vs. post), protein (cricket, pea, whey), and time x protein interaction.

RESULTS: Nuclear magnetic resonance (NMR)-based metabolomics resulted in the annotation and quantification of 25 metabolites in blood, 35 in urine and 21 in muscle tissue. Changes in the muscle metabolome after combined exercise and protein intake indicated effects related to the protein source ingested. Muscle concentrations of leucine, methionine, glutamate and myo-inositol were higher after intake of whey protein compared to both cricket and pea protein. The blood metabolome revealed changes in a more ketogenic direction three hours after exercise reflecting that the trial was conducted after overnight fasting. Urinary concentration of trimethylamine N-oxide was significantly higher after ingestion of cricket than pea and whey protein.

CONCLUSION: The blood, urine and muscle metabolome showed different and supplementary responses to exercise and ingestion of the different protein sources, and in synergy the summarized results provided a more complete picture of the metabolic state of the body.

Original language	English
Article number	98
Journal	Metabolomics
Volume	19
Issue	12
Number of pages	14
ISSN	1573-3882
DOIs	https://doi.org/10.1007/s11306-023-02064-0
Publication status	Published - Dec 2023

Keywords

Blood metabolome
Human metabolome
Insect protein
Muscle metabolome
NMR-based metabolomics
Protein ingestion
Urine metabolome

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10.1007/s11306-023-02064-0

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title = "Acute changes in the metabolome following resistance exercise combined with intake of different protein sources (cricket, pea, whey)",

abstract = "INTRODUCTION: Separately, both exercise and protein ingestion have been shown to alter the blood and urine metabolome. This study goes a step further and examines changes in the metabolome derived from blood, urine and muscle tissue extracts in response to resistance exercise combined with ingestion of three different protein sources.METHODS: In an acute parallel study, 52 young males performed one-legged resistance exercise (leg extension, 4 × 10 repetitions at 10 repetition maximum) followed by ingestion of either cricket (insect), pea or whey protein (0.25 g protein/kg fat free mass). Blood and muscle tissue were collected at baseline and three hours after protein ingestion. Urine was collected at baseline and four hours after protein ingestion. Mixed-effects analyses were applied to examine the effect of the time (baseline vs. post), protein (cricket, pea, whey), and time x protein interaction.RESULTS: Nuclear magnetic resonance (NMR)-based metabolomics resulted in the annotation and quantification of 25 metabolites in blood, 35 in urine and 21 in muscle tissue. Changes in the muscle metabolome after combined exercise and protein intake indicated effects related to the protein source ingested. Muscle concentrations of leucine, methionine, glutamate and myo-inositol were higher after intake of whey protein compared to both cricket and pea protein. The blood metabolome revealed changes in a more ketogenic direction three hours after exercise reflecting that the trial was conducted after overnight fasting. Urinary concentration of trimethylamine N-oxide was significantly higher after ingestion of cricket than pea and whey protein.CONCLUSION: The blood, urine and muscle metabolome showed different and supplementary responses to exercise and ingestion of the different protein sources, and in synergy the summarized results provided a more complete picture of the metabolic state of the body.",

keywords = "Blood metabolome, Human metabolome, Insect protein, Muscle metabolome, NMR-based metabolomics, Protein ingestion, Urine metabolome",

author = "Lanng, {Sofie Kaas} and Mikkel Oxfeldt and Johansen, {Frank Ted} and Jeyanthini Risikesan and Mette Hansen and Bertram, {Hanne Christine}",

year = "2023",

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doi = "10.1007/s11306-023-02064-0",

language = "English",

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Acute changes in the metabolome following resistance exercise combined with intake of different protein sources (cricket, pea, whey). / Lanng, Sofie Kaas; Oxfeldt, Mikkel; Johansen, Frank Ted et al.
In: Metabolomics, Vol. 19, No. 12, 98, 12.2023.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

TY - JOUR

T1 - Acute changes in the metabolome following resistance exercise combined with intake of different protein sources (cricket, pea, whey)

AU - Lanng, Sofie Kaas

AU - Oxfeldt, Mikkel

AU - Johansen, Frank Ted

AU - Risikesan, Jeyanthini

AU - Hansen, Mette

AU - Bertram, Hanne Christine

PY - 2023/12

Y1 - 2023/12

N2 - INTRODUCTION: Separately, both exercise and protein ingestion have been shown to alter the blood and urine metabolome. This study goes a step further and examines changes in the metabolome derived from blood, urine and muscle tissue extracts in response to resistance exercise combined with ingestion of three different protein sources.METHODS: In an acute parallel study, 52 young males performed one-legged resistance exercise (leg extension, 4 × 10 repetitions at 10 repetition maximum) followed by ingestion of either cricket (insect), pea or whey protein (0.25 g protein/kg fat free mass). Blood and muscle tissue were collected at baseline and three hours after protein ingestion. Urine was collected at baseline and four hours after protein ingestion. Mixed-effects analyses were applied to examine the effect of the time (baseline vs. post), protein (cricket, pea, whey), and time x protein interaction.RESULTS: Nuclear magnetic resonance (NMR)-based metabolomics resulted in the annotation and quantification of 25 metabolites in blood, 35 in urine and 21 in muscle tissue. Changes in the muscle metabolome after combined exercise and protein intake indicated effects related to the protein source ingested. Muscle concentrations of leucine, methionine, glutamate and myo-inositol were higher after intake of whey protein compared to both cricket and pea protein. The blood metabolome revealed changes in a more ketogenic direction three hours after exercise reflecting that the trial was conducted after overnight fasting. Urinary concentration of trimethylamine N-oxide was significantly higher after ingestion of cricket than pea and whey protein.CONCLUSION: The blood, urine and muscle metabolome showed different and supplementary responses to exercise and ingestion of the different protein sources, and in synergy the summarized results provided a more complete picture of the metabolic state of the body.

AB - INTRODUCTION: Separately, both exercise and protein ingestion have been shown to alter the blood and urine metabolome. This study goes a step further and examines changes in the metabolome derived from blood, urine and muscle tissue extracts in response to resistance exercise combined with ingestion of three different protein sources.METHODS: In an acute parallel study, 52 young males performed one-legged resistance exercise (leg extension, 4 × 10 repetitions at 10 repetition maximum) followed by ingestion of either cricket (insect), pea or whey protein (0.25 g protein/kg fat free mass). Blood and muscle tissue were collected at baseline and three hours after protein ingestion. Urine was collected at baseline and four hours after protein ingestion. Mixed-effects analyses were applied to examine the effect of the time (baseline vs. post), protein (cricket, pea, whey), and time x protein interaction.RESULTS: Nuclear magnetic resonance (NMR)-based metabolomics resulted in the annotation and quantification of 25 metabolites in blood, 35 in urine and 21 in muscle tissue. Changes in the muscle metabolome after combined exercise and protein intake indicated effects related to the protein source ingested. Muscle concentrations of leucine, methionine, glutamate and myo-inositol were higher after intake of whey protein compared to both cricket and pea protein. The blood metabolome revealed changes in a more ketogenic direction three hours after exercise reflecting that the trial was conducted after overnight fasting. Urinary concentration of trimethylamine N-oxide was significantly higher after ingestion of cricket than pea and whey protein.CONCLUSION: The blood, urine and muscle metabolome showed different and supplementary responses to exercise and ingestion of the different protein sources, and in synergy the summarized results provided a more complete picture of the metabolic state of the body.

KW - Blood metabolome

KW - Human metabolome

KW - Insect protein

KW - Muscle metabolome

KW - NMR-based metabolomics

KW - Protein ingestion

KW - Urine metabolome

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U2 - 10.1007/s11306-023-02064-0

DO - 10.1007/s11306-023-02064-0

M3 - Journal article

C2 - 37999866

SN - 1573-3882

VL - 19

JO - Metabolomics

JF - Metabolomics

IS - 12

M1 - 98

ER -

Acute changes in the metabolome following resistance exercise combined with intake of different protein sources (cricket, pea, whey)

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