TY - JOUR
T1 - Acemannan increased bone surface, bone volume, and bone density in a calvarial defect model in skeletally-mature rats
AU - Godoy, Dyna Jeanne D.
AU - Chokboribal, Jaroenporn
AU - Pauwels, Ruben
AU - Banlunara, Wijit
AU - Sangvanich, Polkit
AU - Jaroenporn, Sukanya
AU - Thunyakitpisal, Pasutha
N1 - Funding Information:
We thank Prof. Suchinda Malaivijitnond, Prof. Dr. Visaka Limwong, Assoc. Prof. Dr. Dolly Methatharathip, and Dr. Kevin A. Tompkins for their valuable suggestions. This study was funded by Chulalongkorn University ; Government Budget, Ratchadaphiseksomphot Endowment Fund Part of the “Strengthen CU's Researcher's Project”, and The 90th Anniversary of Chulalongkorn University, Ratchadapiseksomphot Fund.
Publisher Copyright:
© 2018 Association for Dental Sciences of the Republic of China
PY - 2018/12
Y1 - 2018/12
N2 - Background/purpose: Acemannan, a β-(1–4)-acetylated polymannose extracted from Aloe vera gel, has been proposed as biomaterial for bone regeneration. The aim of this study was to investigate the effect of acemannan in calvarial defect healing. Materials and methods: Acemannan was processed to freeze-dried sponge form and disinfected by UV irradiation. Thirty-five female Sprague–Dawley rats were used in the in vivo study. Seven-mm diameter mid-calvarial defects were created and randomly allocated into blood clot control (C), acemannan 1 mg (A1), 2 mg (A2), 4 mg (A4), and 8 mg (A8) groups (n = 7). After four weeks, the calvarial specimens were subjected to microcomputed tomography (microCT) and histopathological analysis. Results: MicroCT revealed a significant increase in bone surface and bone volume in the A1 and A2 groups, and tissue mineral density in the A4 and A8 groups compared with the control group (p < 0.05). Histologically, the acemannan-treated groups had denser bone matrix compared with the control group. Conclusion: Acemannan is an effective bioactive agent for bone regeneration, enhancing bone growth as assayed in two- and three-dimensions.
AB - Background/purpose: Acemannan, a β-(1–4)-acetylated polymannose extracted from Aloe vera gel, has been proposed as biomaterial for bone regeneration. The aim of this study was to investigate the effect of acemannan in calvarial defect healing. Materials and methods: Acemannan was processed to freeze-dried sponge form and disinfected by UV irradiation. Thirty-five female Sprague–Dawley rats were used in the in vivo study. Seven-mm diameter mid-calvarial defects were created and randomly allocated into blood clot control (C), acemannan 1 mg (A1), 2 mg (A2), 4 mg (A4), and 8 mg (A8) groups (n = 7). After four weeks, the calvarial specimens were subjected to microcomputed tomography (microCT) and histopathological analysis. Results: MicroCT revealed a significant increase in bone surface and bone volume in the A1 and A2 groups, and tissue mineral density in the A4 and A8 groups compared with the control group (p < 0.05). Histologically, the acemannan-treated groups had denser bone matrix compared with the control group. Conclusion: Acemannan is an effective bioactive agent for bone regeneration, enhancing bone growth as assayed in two- and three-dimensions.
KW - Acemannan
KW - Aloe vera
KW - Bone repair
KW - Histopathology
KW - Microcomputed tomography
UR - http://www.scopus.com/inward/record.url?scp=85050132605&partnerID=8YFLogxK
U2 - 10.1016/j.jds.2018.06.004
DO - 10.1016/j.jds.2018.06.004
M3 - Journal article
AN - SCOPUS:85050132605
SN - 1991-7902
VL - 13
SP - 334
EP - 341
JO - Journal of Dental Sciences
JF - Journal of Dental Sciences
IS - 4
ER -