A traditional Chinese medicine formula extracts stimulate proliferation and inhibit mineralization of human mesenchymal stem cells in vitro

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  • Muwan Chen
  • Wenzhou Feng, Denmark
  • Hui Cao, Denmark
  • Lijin Zou, Denmark
  • Chungui Chen, Denmark
  • Anette Baatrup
  • Anna Bay Nielsen, Aarhus Sygehus, Denmark
  • Haisheng Li, Denmark
  • Moustapha Kassem, Denmark
  • Xuenong Zou, Denmark
  • Cody Bünger
  • Interdisciplinary Nanoscience Center
  • Institute of Clinical Medicine
  • The Department of Orthopaedics E, ?AS
  • Ortopædkirurgisk Afdeling E, THG
AIM OF THE STUDY: To investigate the effects of a traditional Chinese medicine (TCM) formula extract, named as ZD-I, on the proliferation and osteogenic differentiation of human mesenchymal stem cells (hMSCs) in vitro. MATERIALS AND METHODS: When hMSCs cultivated in the basal medium with ZD-I, cell viability was assessed by MTT assay and cellular proliferation was assessed by SYBR green I assay. The effects of ZD-I on osteogenic differentiation of hMSCs were assessed by alkaline phosphatase (ALP) activity, mineralization assay and real-time RT-PCR. RESULTS: ZD-I (0.78-100 microg/ml) was non-cytotoxic. The 50% inhibitory concentration (IC50) of hMSCs was 200 microg/ml. ZD-I (0.78-50 microg/ml) stimulated the proliferation of hMSCs. ZD-I did not change ALP activity of hMSCs cultivated in osteogenic medium in the early stage (4 and 7 days), but ZD-I inhibited the mineralization of hMSCs through down-regulation of several osteogenic markers (e.g. osteocalcin, bone morphogenetic protein 2 and osteopontin) in the late stage. CONCLUSIONS: ZD-I stimulate cellular proliferation and decrease the bone mineral deposition of hMSCs. These results suggest ZD-I may play an important therapeutic role in osteoarthritic patients by improving proliferative capacity of hMSCs and inhibiting the mineralization of hMSCs.
Original languageEnglish
JournalJournal of ethnopharmacology
Pages (from-to)75-82
Number of pages7
Publication statusPublished - 2009

    Research areas

  • Alkaline Phosphatase, Cell Differentiation, Cell Proliferation, Culture Media, Gene Expression Regulation, Humans, Medicine, Chinese Traditional, Mesenchymal Stem Cells, Osteoblasts, Plant Extracts

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